Document Detail

Evaluation of rat sperm by flow cytometry: simultaneous analysis of sperm count and sperm viability.
MedLine Citation:
PMID:  9922939     Owner:  NLM     Status:  MEDLINE    
In this study, we conducted a simultaneous analysis of sperm count and viability in rats by flow cytometry (FCM). Epididymal fluids were taken from the caudal epididymis of 12 to 13 week-old Sprague-Dawley rats. The fluids were weighed and mixed with Dulbecco's phosphate buffered saline (D-PBS). Propidium iodide, which can stain only dead sperm, was used to distinguish viable and dead sperm. The sperm count and viability analyzed by FCM were 1.28 x 10(6)/mg and 78.0%, respectively. These values were consistent with the corresponding values (1.39 x 10(6)/mg and 81.0%) that were directly determined microscopically in the fluids of the same sample. In addition, when the original mixture containing sperm was diluted two times and four times with D-PBS, or was diluted two times with D-PBS containing only killed sperm, the sperm count and viability determined by FCM also correlated well with the sperm count (r = 0.96, P < 0.01) and sperm motility (r = 0.99, P < 0.01) by direct microscopic observation, respectively. In conclusion, the present flow cytometric analysis would be practical for the simultaneous determination of sperm count and viability in rat epididymal fluids.
T Yamamoto; S Mori; M Yoneyama; M Imanishi; M Takeuchi
Related Documents :
3584619 - Relationship of sire fertility to acrosome-reacted and motile spermatozoa after treatme...
15923029 - Comparison of three different staining methods for the assessment of epididymal red dee...
12333579 - How is postvasectomy sterility confirmed?
19443909 - Considerations in evaluating human spermatogenesis on the basis of total sperm per ejac...
15533339 - Clinical application of sperm-oocyte interaction tests in in vitro fertilization--embry...
20738589 - Sperm design and function in the redside dace clinostomus elongatus.
22591379 - Influence of nanocoated calcium phosphate on two different types of implant surfaces in...
16594359 - Removable partial denture abutments restored with all-ceramic surveyed crowns.
18072029 - Immune response to xenogeneic matrix grafts used in pediatric surgery.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of toxicological sciences     Volume:  23     ISSN:  0388-1350     ISO Abbreviation:  J Toxicol Sci     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-04-14     Completed Date:  1999-04-14     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7805798     Medline TA:  J Toxicol Sci     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  373-8     Citation Subset:  IM    
Yoshitomi Safety Evaluation Laboratory, Yoshitomi Pharmaceutical Industries, Ltd., Fukuoka, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Survival
Flow Cytometry / methods*
Propidium / diagnostic use
Rats, Sprague-Dawley
Semen / cytology
Sperm Count*
Sperm Motility
Spermatozoa / physiology*
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Influences of alkaline ionized water on milk yield, body weight of offspring and perinatal dam in ra...
Next Document:  Methylmercury-induced microtubule depolymerization leads to inhibition of tubulin synthesis.