Document Detail


Evaluation of rat sperm by flow cytometry: simultaneous analysis of sperm count and sperm viability.
MedLine Citation:
PMID:  9922939     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we conducted a simultaneous analysis of sperm count and viability in rats by flow cytometry (FCM). Epididymal fluids were taken from the caudal epididymis of 12 to 13 week-old Sprague-Dawley rats. The fluids were weighed and mixed with Dulbecco's phosphate buffered saline (D-PBS). Propidium iodide, which can stain only dead sperm, was used to distinguish viable and dead sperm. The sperm count and viability analyzed by FCM were 1.28 x 10(6)/mg and 78.0%, respectively. These values were consistent with the corresponding values (1.39 x 10(6)/mg and 81.0%) that were directly determined microscopically in the fluids of the same sample. In addition, when the original mixture containing sperm was diluted two times and four times with D-PBS, or was diluted two times with D-PBS containing only killed sperm, the sperm count and viability determined by FCM also correlated well with the sperm count (r = 0.96, P < 0.01) and sperm motility (r = 0.99, P < 0.01) by direct microscopic observation, respectively. In conclusion, the present flow cytometric analysis would be practical for the simultaneous determination of sperm count and viability in rat epididymal fluids.
Authors:
T Yamamoto; S Mori; M Yoneyama; M Imanishi; M Takeuchi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of toxicological sciences     Volume:  23     ISSN:  0388-1350     ISO Abbreviation:  J Toxicol Sci     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-04-14     Completed Date:  1999-04-14     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7805798     Medline TA:  J Toxicol Sci     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  373-8     Citation Subset:  IM    
Affiliation:
Yoshitomi Safety Evaluation Laboratory, Yoshitomi Pharmaceutical Industries, Ltd., Fukuoka, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Survival
Flow Cytometry / methods*
Male
Propidium / diagnostic use
Rats
Rats, Sprague-Dawley
Semen / cytology
Sperm Count*
Sperm Motility
Spermatozoa / physiology*
Chemical
Reg. No./Substance:
36015-30-2/Propidium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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