Document Detail


Evaluation of radioimmunoassays: comparison of dose interpolation calculations by four parameter logistic and spline functions.
MedLine Citation:
PMID:  3546584     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two computerized methods for dose interpolation calculation were compared. Generated data sets with a known coefficient of variation as well as laboratory RIA data were analysed. The four parameter logistic method, which is based on an approximation of the mass action law, performed better than the Spline method, a procedure which makes no a priori assumptions about the data. Correct weighting of the data was important for obtaining satisfactory fits. The determination of the response error relationship proved to be the most satisfactory approach in obtaining suitable weighting factors.
Authors:
M Kraupp; R März; E Legenstein; B Knerer; T Szekeres
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical chemistry and clinical biochemistry. Zeitschrift für klinische Chemie und klinische Biochemie     Volume:  24     ISSN:  0340-076X     ISO Abbreviation:  J. Clin. Chem. Clin. Biochem.     Publication Date:  1986 Dec 
Date Detail:
Created Date:  1987-03-30     Completed Date:  1987-03-30     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  7701860     Medline TA:  J Clin Chem Clin Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  1023-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Biopterin / analogs & derivatives,  blood
Dose-Response Relationship, Drug
Humans
Insulin / blood
Mathematical Computing*
Monte Carlo Method
Neopterin
Numerical Analysis, Computer-Assisted*
Quality Control
Radioimmunoassay*
Chemical
Reg. No./Substance:
11061-68-0/Insulin; 22150-76-1/Biopterin; 670-65-5/Neopterin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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