Document Detail


Evaluation of rK-39 strip test using urine for diagnosis of visceral leishmaniasis in an endemic region of India.
MedLine Citation:
PMID:  23149580     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The definitive diagnosis of visceral leishmaniasis (VL) requires invasive procedures for demonstration of parasites in tissue smear or culture. These procedures need expertise and laboratory supports and cannot be performed in the field. The aim of the present study was to evaluate the existing rK-39 immunochromatographic nitrocellulose strips test (ICT) with some modification in human urine for diagnosis of VL. The test was performed on both sera and urine samples on the same 786 subjects (365 confirmed VL and 421 control subjects). The sensitivity of the rK-39 ICT in serum was 100%, whereas the specificity was 93.8%, 100%, and 96.2% in healthy controls from endemic, non-endemic, and other infectious diseases, respectively. However, in urine samples, the test showed 96.1% sensitivity and 100% specificity. Considering sensitivity and feasibility of the test in the field, rK-39 ICT using urine samples can be an alternative to conventional invasive VL diagnosis.
Authors:
Dharmendra Singh; Krishna Pandey; Vidya Nand Rabi Das; Sushmita Das; Neena Verma; Alok Ranjan; Sekhar Chandra Lal; Kamal Roshan Topno; Shubhankar Kumar Singh; Rakesh Bihari Verma; Ashish Kumar; Abul Hasan Sardar; Bidyut Purkait; Pradeep Das
Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2012-11-13
Journal Detail:
Title:  The American journal of tropical medicine and hygiene     Volume:  88     ISSN:  1476-1645     ISO Abbreviation:  Am. J. Trop. Med. Hyg.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-07     Completed Date:  2013-04-01     Revised Date:  2014-02-14    
Medline Journal Info:
Nlm Unique ID:  0370507     Medline TA:  Am J Trop Med Hyg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  222-6     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Antibodies, Protozoan / blood
Case-Control Studies
Child
Child, Preschool
Female
Humans
Immunochromatography
India / epidemiology
Leishmania / isolation & purification,  pathogenicity
Leishmaniasis, Visceral / blood,  diagnosis*,  epidemiology*,  urine*
Male
Middle Aged
Sensitivity and Specificity
Young Adult
Chemical
Reg. No./Substance:
0/Antibodies, Protozoan
Comments/Corrections

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