Document Detail

Evaluation of quantitative recovery methods for Listeria monocytogenes applied to stainless steel.
MedLine Citation:
PMID:  17580634     Owner:  NLM     Status:  MEDLINE    
The ability of Listeria monocytogenes to attach to various food contact surfaces, such as stainless steel, polypropylene, and rubber compounds, is well documented. The retention of these or other pathogenic bacteria on food contact surfaces increases the risk of transmission to food products. The objective of this study was to compare several methods for quantitative recovery of Listeria monocytogenes from stainless steel surfaces. A cocktail of 4 serotypes of Listeria monocytogenes mixed in equivalent concentrations was inoculated onto type 304 stainless steel coupons in a 2 x 2 cm area. After 1 h exposure, coupons were sampled by one of the following methods: (1) swabbing with a premoistened Dacron swab; (2) rinsing with phosphate-buffered saline; (3) direct contact onto tryptic soy agar containing 0.6% yeast extract (TSA + YE) plates for 10 s; (4) sonication in an ultrasonic water bath (40 kHz); (5) contact with the bristles of a sonicating brush head for 1 min; and (6) indirect contact (2-4 mm distance) with a sonicating brush head for 1 min. The 3 sonication methods yielded higher recovery than the other 3 methods (P < 0.05). Brushing the coupons with the sonicating brush head (contact or noncontact) yielded a recovery level of about 60%. The lowest cell recovery (about 20%) was observed with the swab and direct agar contact methods. After a 12 h exposure, recoveries ranged from 17.4 (brush contact method) to 2% (swab method).
David Kang; Joseph D Eifert; Robert C Williams; Steven Pao
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  90     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2007 May-Jun
Date Detail:
Created Date:  2007-06-21     Completed Date:  2007-07-20     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  810-6     Citation Subset:  IM    
Virginia Tech, Department of Food Science and Technology (0418), Blacksburg, VA 24061, USA.
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MeSH Terms
Actinomyces / metabolism
Agar / chemistry
Chemistry Techniques, Analytical / methods*
Equipment Design
Evaluation Studies as Topic
Listeria monocytogenes / genetics*
Microbiological Techniques*
Phosphates / chemistry
Research Design
Stainless Steel
Time Factors
Reg. No./Substance:
0/Phosphates; 12597-68-1/Stainless Steel; 9002-18-0/Agar

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