Document Detail

Evaluation of process-induced dimensional changes in the membrane structure of biological cells using impedance measurement.
MedLine Citation:
PMID:  12052078     Owner:  NLM     Status:  MEDLINE    
The impact of high intensity electric field pulses, high hydrostatic pressure, and freezing-thawing on local structural changes of the membrane was determined for potato, sugar beet tissue, and yeast suspensions. On the basis of the electrophysical model of cell systems in biological tissues and suspensions, a method was derived for determining the extent of local damage of cell membranes. The method was characterized by an accurate and rapid on-line determination of frequency-dependent electrical conductivity properties from which information on microscopic events on cellular level may be deduced. Evaluation was based on the measurement of the relative change in the sample's impedance at characteristically low (f(l)) and high (f(h)) frequencies within the beta-dispersion range. For plant and animal cells the characteristic frequencies were f(l) approximately 5 kHz and f(h) > 5 MHz and for yeast cells in the range f(l) approximately 50 kHz and f(h) > 25 MHz. The observed phenomena were complex. The identification of the underlying mechanisms required consideration of the time-dependent nature of the processing effects and stress reactions of the biological systems, which ranged from seconds to several hours. A very low but significantly detectable membrane damage (0.004% of the total area) was found after high hydrostatic pressure treatment of potato tissue at 200 MPa. The membrane rupture in plant tissue cells was higher after freezing and subsequent thawing (0.9% of total area for potato cells and 0.05-0.07% for sugar beet cells determined immediately after thawing), which increased substantially during the next 2 h.
Alexander Angersbach; Volker Heinz; Dietrich Knorr
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology progress     Volume:  18     ISSN:  8756-7938     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    2002 May-Jun
Date Detail:
Created Date:  2002-06-07     Completed Date:  2002-12-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  597-603     Citation Subset:  IM    
Department of Food Biotechnology and Food Process Engineering, Berlin University of Technology, Königin-Luise-Str. 22, D-14195 Berlin, Germany.
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MeSH Terms
Cell Membrane*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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