Document Detail

Evaluation of the pro-angiogenic effect of factor XIII in heterotopic mouse heart allografts and FXIII-deficient mice.
MedLine Citation:
PMID:  16525585     Owner:  NLM     Status:  MEDLINE    
Thrombin-activated Factor XIII (FXIIIa), a plasma transglutaminase, stabilizes fibrin clots by crosslinking fibrin chains. FXIIIa was previously shown by us to exhibit proangiogenic activity associated with downregulation of thrombospondin-1, phosphorylation of vascular endothelial growth factor receptor 2 (VEGFR-2), and upregulation of c-Jun. In the current study, we evaluated the proangiogenic effect of FXIIIa in two murine models: a neonatal heterotopic cardiac allograft model in normal mice, and a Matrigel plug model in FXIII-deficient mice. In the neonatal cardiac allograft model, the number of new vessels as well as graft viability (contractile performance) was significantly higher in FXIIIa-injected animals than in controls. A significant increase in the level of c-Jun mRNA and a significant decrease in the level of TSP-1 mRNA were observed in heart allografts treated with FXIIIa. A marked decrease in TSP-1 protein expression was observed within the endothelial cells of hearts treated with FXIIIa. In the Matrigel plug model, FXIII-deficient mice showed a significantly decreased number of new vessels compared to that of the control mice, and the number of vessels almost reached normal levels following addition of FXIIIa. The results of this study provide substantial in vivo evidence for the proangiogenic activity of FXIIIa.
Rima Dardik; Jonathan Leor; Ehud Skutelsky; David Castel; Radka Holbova; Ginette Schiby; Aviv Shaish; Gerhard Dickneite; Joseph Loscalzo; Aida Inbal
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Thrombosis and haemostasis     Volume:  95     ISSN:  0340-6245     ISO Abbreviation:  Thromb. Haemost.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-09     Completed Date:  2006-04-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7608063     Medline TA:  Thromb Haemost     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  546-50     Citation Subset:  IM    
Institute of Thrombosis and Hemostasis, Sheba Medical Center, Tel Hashomer, Israel.
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MeSH Terms
Angiogenesis Inducing Agents / pharmacology*
Animals, Newborn
Coronary Vessels / drug effects
Dose-Response Relationship, Drug
Drug Combinations
Ear, External
Factor XIII / genetics
Factor XIII Deficiency / genetics*
Factor XIIIa / pharmacology*
Graft Survival / drug effects
Heart Transplantation*
Mice, Inbred BALB C
Mice, Knockout
Models, Animal
Myocardium / metabolism
Neovascularization, Physiologic / drug effects*
Proto-Oncogene Proteins c-jun / genetics,  metabolism
RNA, Messenger / metabolism
Thrombospondin 1 / genetics,  metabolism
Transplantation, Heterotopic*
Reg. No./Substance:
0/Angiogenesis Inducing Agents; 0/Drug Combinations; 0/Laminin; 0/Proteoglycans; 0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger; 0/Thrombospondin 1; 119978-18-6/matrigel; 9007-34-5/Collagen; 9013-56-3/Factor XIII; EC XIIIa

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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