Document Detail


Evaluation of porcine ileum models of enterocyte infection by Lawsonia intracellularis.
MedLine Citation:
PMID:  16639950     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The early interaction of Lawsonia intracellularis with host cells was examined with the use of porcine ileum models. Two conventional swine were anesthetized, and ligated ileum loops were prepared during abdominal surgery. The loops were inoculated with 108 L. intracellularis or saline. After 60 min, samples of each loop were processed for routine histologic and electron microscopic study. Histologic and ultrathin sections of all the loops appeared normal, with no apposition of bacteria and host cells or bacterial entry events in any loop. Portions of ileum from a single gnotobiotic piglet were introduced as xenografts into the subcutis of each flank of 5 weaned mice with severe combined immunodeficiency disease. After 4 wk, 108 L. intracellularis were inoculated into each of 4 viable xenografts with a sterile needle; the other 3 viable xenografts received saline. Histologic and ultrathin sections of all the xenografts 3 wk after inoculation showed relatively normal porcine intestinal architecture, with normal crypts, crypt cell differentiation, and low villous structures; the xenografts treated with the bacteria also showed intracytoplasmic L. intracellularis within crypt and villous epithelial cells. Thus, entry of L. intracellularis into target epithelial cells and multiplication may not be sufficient alone to directly cause cell proliferation. A proliferative response may require active division of crypt cells and differentiation in conjunction with L. intracellularis growth.
Authors:
Steven McOrist; Connie J Gebhart; Brad T Bosworth
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire     Volume:  70     ISSN:  0830-9000     ISO Abbreviation:  Can. J. Vet. Res.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-27     Completed Date:  2006-06-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8607793     Medline TA:  Can J Vet Res     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  155-9     Citation Subset:  IM    
Affiliation:
Tufts University School of Veterinary Medicine, 200 Westboro Road, North Grafton, Massachusetts 01536, USA. steven.mcorist@nottingham.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division
Desulfovibrionaceae Infections / microbiology,  pathology,  veterinary*
Disease Models, Animal
Enterocytes / microbiology*,  physiology*
Erythrocytes / microbiology*
Ileum* / cytology,  microbiology,  pathology,  ultrastructure
Lawsonia Bacteria / pathogenicity*
Mice
Microscopy, Electron, Transmission / veterinary
Swine
Swine Diseases
Transplantation, Heterologous / veterinary
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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