Document Detail

Evaluation of the porcine gastric mucin binding assay for high pressure inactivation studies using murine norovirus and Tulane virus.
MedLine Citation:
PMID:  25362063     Owner:  NLM     Status:  Publisher    
We compared high hydrostatic pressure (HHP) inactivation results of murine norovirus (MNV-1) and Tulane virus (TV) obtained through the porcine gastric mucin binding assay (referred to as PGM-MB/PCR assay) and plaque assay and evaluated HHP inactivation of human norovirus (HuNoV) GI.1 and GII.4 strains using the PGM-MB/PCR assay. Viruses were treated at different pressure levels for 2 min at 4 or 21°C in culture media of neutral pH and in culture media of pH 4 at 21°C. The log reductions of infectious virus particles of MNV-1 and TV caused by HHP were assessed using the PGM-MB/PCR and plaque assays while the log reductions of HuNoVs were assessed by the PGM-MB/PCR assay only. For TV and MNV-1, the two pressure inactivation curves obtained using the plaque and PGM-MB/PCR assays were almost identical at ≤ ∼2-log reduction levels regardless the treatment temperature and pH. Further increasing the pressure level over the ∼2 log reduction level resulted in higher log reductions of TV and MNV-1 assessed by the plaque assay, but did not increase the log reductions assessed by the PGM-MB/PCR assay. HHP treatments could achieve maximums of ∼3 and 3.5 log reductions of GI.1 and GII.4 assessed by PGM-MB/PCR assay, respectively. Based on these results, it can be reasonably concluded that the PGM-MB/PCR assay would be very likely able to estimate HHP inactivation of HuNoV at ≤ 2 log-reduction levels. It would also likely to conservatively quantify HHP inactivation of GI.1 strain at 2-3 log-reduction levels and GII.4 strain at 2-3.5 log-reduction levels.
Xinhui Li; Haiqiang Chen
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-10-31
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  -     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2014 Oct 
Date Detail:
Created Date:  2014-11-1     Completed Date:  -     Revised Date:  2014-11-2    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014, American Society for Microbiology. All Rights Reserved.
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