| Evaluation of polymerase chain reaction using protein b primers for rapid diagnosis of tuberculous meningitis. | |
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MedLine Citation:
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PMID: 21045496 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background: Rapid and specific diagnosis of tubercular meningitis (TBM) is of utmost importance. Aim: To evaluate polymerase chain reaction (PCR) using protein b primers directed against M. tuberculosis for the diagnosis of tuberculous meningitis. Materials and Methods: PCR using protein b primers was performed in ten patients with confirmed TBM (culture positive), 60 patients with clinically suspected TBM and 40 patients with no TBM (control group). Results: Protein b PCR had a sensitivity of 90% and a specificity of 100% in patients with confirmed TBM. In 60 clinically diagnosed TBM patients, protein b PCR was positive in 49 (81.7%) patients. The overall sensitivity of microscopy, culture and PCR using protein b primers was 1.4%, 14.3%, and 82.8% and specificity was 100%, 100%, and 100% respectively. Conclusion: Protein b PCR is valuable in rapid diagnosis of TBM. |
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Authors:
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Kusum Sharma; Aman Sharma; Malkit Singh; Pallab Ray; Ritika Dandora; Shiv K Sharma; Manish Modi; Sudesh Prabhakar; Meera Sharma |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Neurology India Volume: 58 ISSN: 0028-3886 ISO Abbreviation: Neurol India Publication Date: 2010 Sep-Oct |
Date Detail:
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Created Date: 2010-11-03 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0042005 Medline TA: Neurol India Country: India |
Other Details:
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Languages: eng Pagination: 727-31 Citation Subset: IM |
Affiliation:
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Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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