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Evaluation of polymerase chain reaction using protein b primers for rapid diagnosis of tuberculous meningitis.
MedLine Citation:
PMID:  21045496     Owner:  NLM     Status:  In-Data-Review    
Background: Rapid and specific diagnosis of tubercular meningitis (TBM) is of utmost importance. Aim: To evaluate polymerase chain reaction (PCR) using protein b primers directed against M. tuberculosis for the diagnosis of tuberculous meningitis. Materials and Methods: PCR using protein b primers was performed in ten patients with confirmed TBM (culture positive), 60 patients with clinically suspected TBM and 40 patients with no TBM (control group). Results: Protein b PCR had a sensitivity of 90% and a specificity of 100% in patients with confirmed TBM. In 60 clinically diagnosed TBM patients, protein b PCR was positive in 49 (81.7%) patients. The overall sensitivity of microscopy, culture and PCR using protein b primers was 1.4%, 14.3%, and 82.8% and specificity was 100%, 100%, and 100% respectively. Conclusion: Protein b PCR is valuable in rapid diagnosis of TBM.
Kusum Sharma; Aman Sharma; Malkit Singh; Pallab Ray; Ritika Dandora; Shiv K Sharma; Manish Modi; Sudesh Prabhakar; Meera Sharma
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neurology India     Volume:  58     ISSN:  0028-3886     ISO Abbreviation:  Neurol India     Publication Date:    2010 Sep-Oct
Date Detail:
Created Date:  2010-11-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0042005     Medline TA:  Neurol India     Country:  India    
Other Details:
Languages:  eng     Pagination:  727-31     Citation Subset:  IM    
Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
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