| Evaluation of poly(ADP-ribose) polymerase cleavage (cPARP) in ejaculated human sperm fractions after induction of apoptosis. | |
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MedLine Citation:
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PMID: 18501896 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To examine the presence of cleaved poly(ADP-ribose) polymerase(s) (cPARP) in ejaculated spermatozoa and determine cPARP levels following exposure to chemical or oxidative stress. DESIGN: Prospective pilot study. SETTING: Tertiary care academic hospital. PATIENT(S): Eight healthy men. INTERVENTION(S): Semen specimens were collected, prepared with double-density-gradient centrifugation, and divided into control, hydrogen peroxide (H(2)O(2)), H(2)O(2) + 3-aminobenzamide (3-ABA), staurosporine (STS), and STS + 3-ABA treated groups. MAIN OUTCOME MEASURE(S): Cleaved PARP and apoptosis markers by flow cytometry. RESULT(S): Cleaved PARP was detected in both neat and mature fractions. The cPARP levels were similar in both mature and immature spermatozoa. In combined mature and immature fractions, a higher percentage of late apoptotic sperm was seen in STS + 3-ABA versus STS. Higher levels of late apoptotic spermatozoa were seen in immature versus mature fractions within STS and STS + 3-ABA groups. Lower levels of cPARP were seen in immature versus mature fractions in H(2)O(2) and H(2)O(2) + 3-ABA treated groups. Cleaved PARP was related to activated caspase-3. CONCLUSION(S): Cleaved PARP is present in ejaculated human spermatozoa. Poly(ADP-ribose) polymerase inhibitors may play a different role in chemical versus oxidative stress-induced sperm damage. |
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Authors:
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Reda Z Mahfouz; Rakesh K Sharma; Kerstin Poenicke; Rajesh Jha; Uwe Paasch; Sonja Grunewald; Ashok Agarwal |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-05-23 |
Journal Detail:
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Title: Fertility and sterility Volume: 91 ISSN: 1556-5653 ISO Abbreviation: Fertil. Steril. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-28 Completed Date: 2009-05-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: United States |
Other Details:
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Languages: eng Pagination: 2210-20 Citation Subset: IM |
Affiliation:
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Reproductive Research Center, Glickman Urological and Kidney Institute, Department of Obstetrics and Gynecology, Cleveland Clinic, Cleveland, Ohio 44195, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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physiology* Benzamides / pharmacology Centrifugation, Density Gradient DNA Damage / drug effects Ejaculation Flow Cytometry Humans Hydrogen Peroxide / pharmacology Hydrolysis In Situ Nick-End Labeling Male Oxidative Stress Poly(ADP-ribose) Polymerases / drug effects, metabolism* Semen / cytology, drug effects Spermatozoa / cytology, enzymology*, physiology Staurosporine / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Benzamides; 3544-24-9/3-aminobenzamide; 62996-74-1/Staurosporine; 7722-84-1/Hydrogen Peroxide; EC 2.4.2.30/Poly(ADP-ribose) Polymerases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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