Document Detail


Evaluation of a point-of-care test for canine C-reactive protein.
MedLine Citation:
PMID:  21827518     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: In veterinary medicine, there is increasing interest in measuring C-reactive protein (CRP) as a tool for diagnosis and monitoring of inflammatory diseases. Reported CRP concentrations for healthy dogs have ranged from 0 to 8.9 mg/L.
OBJECTIVES: The aims of this study were to evaluate a canine-specific point-of-care (POC) lateral flow immunoassay for qualitative CRP measurement in healthy and diseased dogs and to compare results with those obtained by a quantitative ELISA.
METHODS: Blood samples from 73 client-owned dogs were available for testing: 16 healthy dogs and 57 dogs with a variety of infectious, inflammatory, or neoplastic diseases. CRP was measured in heparinized whole blood samples and serum with the TECO medical Dog CRP-visual POC test. A red line develops in the POC device if CRP is ≥ 5 mg/L, and results are scored as negative or positive. An ELISA validated previously for canine serum was used as the reference method. Results: For all dogs, serum CRP concentrations measured by the ELISA ranged from 0.1 to ≥ 350 mg/L (median = 38 mg/L). Percentages of the CRP POC test results that agreed with the ELISA results were 98.6% for whole blood and 97.3% for serum samples. For serum samples, sensitivity of the POC test was 96.4% and specificity was 81.3%. For whole blood, sensitivity was 94.7% and specificity was 93.8%.
CONCLUSIONS: The POC test had very good agreement with the ELISA test and had high sensitivity and specificity; therefore, it can be used as a qualitative test to screen for increases in CRP concentrations.
Authors:
Hanna D Plickert; Ralf Einspanier; Gisela Arndt; Leo Brunnberg; Barbara Kohn
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2011-08-09
Journal Detail:
Title:  Veterinary clinical pathology / American Society for Veterinary Clinical Pathology     Volume:  40     ISSN:  1939-165X     ISO Abbreviation:  Vet Clin Pathol     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-09-02     Completed Date:  2012-04-17     Revised Date:  2012-10-04    
Medline Journal Info:
Nlm Unique ID:  9880575     Medline TA:  Vet Clin Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  384-8     Citation Subset:  IM    
Copyright Information:
©2011 American Society for Veterinary Clinical Pathology.
Affiliation:
Small Animal Clinic, Faculty of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / analysis,  blood
C-Reactive Protein / analysis*
Case-Control Studies
Dog Diseases / blood*,  diagnosis
Dogs / blood*
Enzyme-Linked Immunosorbent Assay / veterinary
Female
Immunoassay / methods,  veterinary*
Inflammation / blood*,  diagnosis
Male
Point-of-Care Systems
Reproducibility of Results
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Biological Markers; 9007-41-4/C-Reactive Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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