| Evaluation of a point-of-care test for canine C-reactive protein. | |
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MedLine Citation:
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PMID: 21827518 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: In veterinary medicine, there is increasing interest in measuring C-reactive protein (CRP) as a tool for diagnosis and monitoring of inflammatory diseases. Reported CRP concentrations for healthy dogs have ranged from 0 to 8.9 mg/L. OBJECTIVES: The aims of this study were to evaluate a canine-specific point-of-care (POC) lateral flow immunoassay for qualitative CRP measurement in healthy and diseased dogs and to compare results with those obtained by a quantitative ELISA. METHODS: Blood samples from 73 client-owned dogs were available for testing: 16 healthy dogs and 57 dogs with a variety of infectious, inflammatory, or neoplastic diseases. CRP was measured in heparinized whole blood samples and serum with the TECO medical Dog CRP-visual POC test. A red line develops in the POC device if CRP is ≥ 5 mg/L, and results are scored as negative or positive. An ELISA validated previously for canine serum was used as the reference method. Results: For all dogs, serum CRP concentrations measured by the ELISA ranged from 0.1 to ≥ 350 mg/L (median = 38 mg/L). Percentages of the CRP POC test results that agreed with the ELISA results were 98.6% for whole blood and 97.3% for serum samples. For serum samples, sensitivity of the POC test was 96.4% and specificity was 81.3%. For whole blood, sensitivity was 94.7% and specificity was 93.8%. CONCLUSIONS: The POC test had very good agreement with the ELISA test and had high sensitivity and specificity; therefore, it can be used as a qualitative test to screen for increases in CRP concentrations. |
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Authors:
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Hanna D Plickert; Ralf Einspanier; Gisela Arndt; Leo Brunnberg; Barbara Kohn |
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Publication Detail:
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Type: Evaluation Studies; Journal Article Date: 2011-08-09 |
Journal Detail:
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Title: Veterinary clinical pathology / American Society for Veterinary Clinical Pathology Volume: 40 ISSN: 1939-165X ISO Abbreviation: Vet Clin Pathol Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-09-02 Completed Date: 2012-04-17 Revised Date: 2012-10-04 |
Medline Journal Info:
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Nlm Unique ID: 9880575 Medline TA: Vet Clin Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 384-8 Citation Subset: IM |
Copyright Information:
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©2011 American Society for Veterinary Clinical Pathology. |
Affiliation:
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Small Animal Clinic, Faculty of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers / analysis, blood C-Reactive Protein / analysis* Case-Control Studies Dog Diseases / blood*, diagnosis Dogs / blood* Enzyme-Linked Immunosorbent Assay / veterinary Female Immunoassay / methods, veterinary* Inflammation / blood*, diagnosis Male Point-of-Care Systems Reproducibility of Results Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 9007-41-4/C-Reactive Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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