Document Detail


Evaluation of podocyte lesion in patients with diabetic nephropathy: Wilms' tumor-1 protein used as a podocyte marker.
MedLine Citation:
PMID:  19969384     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: The reduction of podocyte number and density per glomerulus has been linked to the development of proteinuria and the progression of disease in patients with diabetic nephropathy (DN). However, it has been recognized that measurement of podocyte number by light microscope is quite difficult because of the complexity of both podocyte and glomerular structure, which is not suitable for clinical research. In our research institute, we used WT1 as podocyte marker to evaluate the podocyte lesion. METHODS: In our experiment, we selected the C-terminal antibody of WT1 to stain the nuclei and the N-terminal antibody of WT1 to stain the cytoplasma of podocytes. Forty patients were enrolled with type 2 diabetes and proven to have DN by renal biopsy analysis. DN patients were classified into three groups based on the degree of proteinuria: microalbuminuria (n=10, 30-300mg/24h), overt proteinuria (n=15, 0.5-3.5g/24h), and heavy proteinuria (n=15, >3.5g/24h). RESULTS: The results demonstrated that the podocyte number was markedly decreased in patients with DN (30-51% reduction). There was a significant negative correlation between the proteinuria and both podocyte density and number. The cover area density of podocyte cytoplasma in glomerulus was also significantly decreased in all DN patients (39-80% reduction). A significant inverse correlation was observed between the cover area density and the degree of proteinuria. The correlation coefficient (r=-0.85) was much higher than that between proteinuria and podocyte density (r=-0.56) or podocyte number (r=-0.36). CONCLUSION: In conclusion, podocyte damage occurred in patients with DN, even in the early stage and became more dramatic during the course of proteinuria progression. WT1 staining, using the polyclonal antibody to stain the nuclei and monoclonal antibody to stain the cytoplasma of podocytes together, is a valuable alternative technique in the study of podocyte injury.
Authors:
Jian Su; Shi-Jun Li; Zhao-Hong Chen; Cai-Hong Zeng; Hong Zhou; Lei-Shi Li; Zhi-Hong Liu
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Publication Detail:
Type:  Journal Article     Date:  2009-12-06
Journal Detail:
Title:  Diabetes research and clinical practice     Volume:  87     ISSN:  1872-8227     ISO Abbreviation:  Diabetes Res. Clin. Pract.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-04-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8508335     Medline TA:  Diabetes Res Clin Pract     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  167-75     Citation Subset:  IM    
Copyright Information:
2009 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Research Institute of Nephrology, Jinling Hospital, Nanjing University School of Medicine, China.
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MeSH Terms
Descriptor/Qualifier:
Adult
Albuminuria / metabolism,  pathology
Biological Markers / metabolism
Biopsy
Blood Glucose / metabolism
Cell Count
Creatinine / blood
Cytoplasm / metabolism,  pathology
Diabetes Mellitus, Type 2 / complications,  metabolism,  pathology
Diabetic Nephropathies / blood,  metabolism,  pathology*
Hemoglobin A, Glycosylated / metabolism
Humans
Kidney Glomerulus / metabolism,  pathology
Middle Aged
Nuclear Proteins / metabolism*
Podocytes / metabolism,  pathology*
Proteinuria / metabolism,  pathology
WT1 Proteins / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Blood Glucose; 0/Hemoglobin A, Glycosylated; 0/Nuclear Proteins; 0/WT1 Proteins; 0/WTAP protein, human; 60-27-5/Creatinine

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