| Evaluation of the permeation of peat substances through human skin in vitro. | |
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MedLine Citation:
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PMID: 12593947 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Peat and various peat extracts have been successfully applied for a variety of clinical indications. Quite apart from the physico-thermal effects, new studies point towards the so-called "chemical effects" of peat containing substances. These effects include a stimulatory response of the spontaneous contractile activity (SCA) of smooth muscle (SM) tissue. The effects are, however, dependent on the possible permeability of pharmacologically active substances as naturally occurring ingredients of peat. Since peat is a mixture of various products it is necessary to examine the various peat types based upon their biological activity on SM tissue. In order to unequivocally prove the pharmacological activity of cutaneous peat treatment, in vitro permeation measurements of these actives across excised human skin can be used.HPLC analysis revealed that aqueous peat extracts contain up to 18 fractions of water-soluble compounds of fulvic and ulmic acids. These compounds have been found to have a stimulatory response on the contractile activity of SM tissue. In vitro diffusion studies showed that the permeability of these substances across human full thickness skin (thickness: 200 um(-1)) is highly selective and the resulting stimulatory activity is dependent on the permeated fraction. Especially, the HPLC fractions 7-11 and 14 are able to permeate human skin. Fractions 7-11 show a moderate stimulatory effect of SCA on SM for more than 90 min whereas fraction 14 shows the strongest stimulatory effect which was, however, suppressed after 87 min. These results show that the cutaneous therapy with peat treatment results in transcutaneaous permeation of biologically active fulvic and ulmic acid derivatives explaining the additional "chemical" effect of peat treatment in clinical practice. |
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Authors:
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André M Beer; H E Junginger; J Lukanov; P Sagorchev |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: International journal of pharmaceutics Volume: 253 ISSN: 0378-5173 ISO Abbreviation: Int J Pharm Publication Date: 2003 Mar |
Date Detail:
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Created Date: 2003-02-20 Completed Date: 2003-09-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7804127 Medline TA: Int J Pharm Country: Netherlands |
Other Details:
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Languages: eng Pagination: 169-75 Citation Subset: IM |
Affiliation:
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Department Naturopathy, Blankenstein Hospital, Hattingen, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Benzopyrans
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isolation & purification,
pharmacokinetics* Chromatography, High Pressure Liquid Humans Humic Substances / isolation & purification, pharmacokinetics* Muscle Contraction / drug effects Muscle, Smooth / drug effects Permeability Skin / metabolism* Skin Absorption Soil* |
| Chemical | |
Reg. No./Substance:
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0/Benzopyrans; 0/Humic Substances; 0/Soil; 479-66-3/fulvic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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