Document Detail


Evaluation of peristalsis in multiple segments of the guinea-pig isolated small intestine: optimisation of tissue use by refined in vitro methodology.
MedLine Citation:
PMID:  15601247     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Peristalsis is the aboral movement by which the intestine propels its contents. Since pharmacological research requires an experimental model with which drug-induced modifications of peristalsis can be reliably quantified, we set out to develop and validate an in vitro method for studying peristalsis in multiple gut segments. In our arrangement, up to four 10cm segments isolated from the guinea-pig jejunum and ileum can be set up in parallel and their lumens perfused. Peristalsis was elicited by pressure-evoked wall distension, and the peristalsis-induced changes in the intraluminal pressure were evaluated with software that determined the peristaltic pressure threshold, the frequency, maximal acceleration and amplitude of the peristaltic waves, and the residual baseline pressure. Validation experiments showed that the peristalsis parameters at baseline and after modification by morphine (0.01-10microM) did not differ between segments from the jejunum and ileum, or between segments examined in a consecutive manner. In conclusion, our work succeeded in optimising the use of the guinea-pig jejunum and ileum for multiple recordings of peristalsis in vitro, and in refining the recording and evaluation of peristaltic motility. This system promises to be particularly useful in the pharmacological screening and testing of drugs which modify peristalsis.
Authors:
Peter Holzer; Anaid Shahbazian; Evelin Painsipp; Akos Heinemann
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Alternatives to laboratory animals : ATLA     Volume:  31     ISSN:  0261-1929     ISO Abbreviation:  Altern Lab Anim     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2004-12-16     Completed Date:  2005-02-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8110074     Medline TA:  Altern Lab Anim     Country:  England    
Other Details:
Languages:  eng     Pagination:  419-27     Citation Subset:  IM    
Affiliation:
Department of Experimental and Clinical Pharmacology, University of Graz, Universitätsplatz 4, 8010 Graz, Austria.
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MeSH Terms
Descriptor/Qualifier:
Animal Use Alternatives / methods*
Animals
Drug Toxicity*
Guinea Pigs
Intestine, Small / drug effects*
Morphine / toxicity
Peristalsis / drug effects*
Tissue Culture Techniques / methods
Toxicity Tests / instrumentation,  methods*
Chemical
Reg. No./Substance:
57-27-2/Morphine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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