| Evaluation of a panel of circulating DNA, RNA and protein potential markers for pathologies of pregnancy. | |
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MedLine Citation:
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PMID: 20374043 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Among markers of pregnancy complications, corticotropin-releasing hormone (CRH) mRNA, long pentraxin 3 (PTX3) protein and fetal and total DNA had been reported to be increased in the plasma of women with overt preeclampsia (PE). We developed an optimized protocol to evaluate whether concentrations of CRH mRNA, PTX3 mRNA and protein, fetal and/or total DNA are increased in fetal growth restriction (FGR), and whether they predict complications of pregnancy. METHODS: The protocol included a preamplification step to enrich rare mRNA species. CRH and PTX3 mRNA, DNA and PTX3 protein were measured in the plasma of women with PE or FGR, in women at risk of developing these pathologies and in healthy women matched for gestational age. RESULTS: CRH mRNA, fetal and/or total DNA and PTX3 protein were significantly increased in women with overt PE when compared to controls. Pregnant women who later developed PE or FGR during pregnancy showed total DNA levels that were significantly increased before the onset of both pathologies, while RNA markers were increased only in women who later developed PE. CONCLUSIONS: Our protocol for plasma RNA quantification may allow for the extension of a panel of predictive markers to be investigated in larger patient cohorts. |
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Authors:
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Silvia Galbiati; Vincenza Causarano; Pamela Pinzani; Salvianti Francesca; Claudio Orlando; Maddalena Smid; Federica Pasi; Maria T Castiglioni; Paolo Cavoretto; Patrizia Rovere-Querini; Silvia Pedroni; Stefano Calza; Maurizio Ferrari; Laura Cremonesi |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical chemistry and laboratory medicine : CCLM / FESCC Volume: 48 ISSN: 1434-6621 ISO Abbreviation: Clin. Chem. Lab. Med. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-03 Completed Date: 2010-09-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9806306 Medline TA: Clin Chem Lab Med Country: Germany |
Other Details:
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Languages: eng Pagination: 791-4 Citation Subset: IM |
Affiliation:
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Genomic Unit for the Diagnosis of Human Pathologies, Center for Genomics, Bioinformatics and Biostatistics, San Raffaele Scientific Institute, Milan, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biological Markers
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blood C-Reactive Protein / genetics Corticotropin-Releasing Hormone / blood, genetics DNA / blood* Female Fetal Growth Retardation / diagnosis* Humans Pre-Eclampsia / diagnosis* Pregnancy RNA, Messenger / blood* Serum Amyloid P-Component / genetics |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/RNA, Messenger; 0/Serum Amyloid P-Component; 148591-49-5/PTX3 protein; 9007-41-4/C-Reactive Protein; 9007-49-2/DNA; 9015-71-8/Corticotropin-Releasing Hormone |
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