| Evaluation of p-naphtholbenzein-beta-D-galactoside as a substrate for bacterial beta-galactosidase. | |
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MedLine Citation:
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PMID: 11097942 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe the synthesis of a new substrate for the detection of beta-galactosidase and evaluate its performance in comparison with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) and cyclohexenoesculetinbeta-D-galactoside (CHE-Gal). Of 206 Enterobacteriaceae strains able to hydrolyze X-Gal, 194 (94.2%) hydrolyzed CHE-Gal and 192 (93.2%) hydrolyzed p-naphtholbenzein-beta-D-galactoside (PNB-Gal). We conclude that PNB-Gal is an effective substrate for the detection of beta-galactosidase. |
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Authors:
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A L James; K F Chilvers; J D Perry; L Armstrong; F K Gould |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 66 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2000 Dec |
Date Detail:
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Created Date: 2001-02-14 Completed Date: 2001-02-15 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 5521-3 Citation Subset: IM |
Affiliation:
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Department of Chemical & Life Sciences, University of Northumbria, Newcastle-upon-Tyne, United Kingdom. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromogenic Compounds
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chemical synthesis Enterobacteriaceae / enzymology* Galactosides* / chemical synthesis Indoles Naphthalenes* / chemical synthesis Substrate Specificity Umbelliferones Yersinia enterocolitica / enzymology beta-Galactosidase / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Chromogenic Compounds; 0/Galactosides; 0/Indoles; 0/Naphthalenes; 0/Umbelliferones; 0/cyclohexenoesculetin-beta-galactoside; 0/p-naphtholbenzeingalactoside; 7240-90-6/5-bromo-4-chloro-3-indolyl beta-galactoside; EC 3.2.1.23/beta-Galactosidase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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