Document Detail

Evaluation of osteonectin as a diagnostic marker of osteogenic bone tumors.
MedLine Citation:
PMID:  1468768     Owner:  NLM     Status:  MEDLINE    
Osteonectin (ON), a 32,000-kd glycoprotein involved in the early steps of mineralization of skeletal tissue, is a recognized differentiation marker of normal osteogenic cells. The expression of ON was evaluated in vitro and in tissue sections by the polyclonal antibody bON II. In different cell cultures immunocytochemistry and molecular biology displayed a nonspecific reaction for the antibody, which showed itself to be useless for the in vitro identification of cells of the osteoblastic lineage. The diagnostic use of bON II antibody was investigated by immunohistochemistry on a series of osteogenic and nonosteogenic bone tumors. A strongly positive stain of the entire neoplastic component of osteosarcoma and osteoblastoma and a weaker stain of the mononuclear component of giant cell tumor and chondroblastoma were observed. On the other hand, stains for chondrosarcoma, Ewing's sarcoma, fibrosarcoma, malignant fibrous histiocytoma, and brown tumor from hyperparathyroidism were entirely negative. Our results indicate that ON may be helpful in the histologic diagnosis of bone tumors, particularly in differentiating small cell osteosarcoma from other small round cell tumors.
M Serra; M C Morini; K Scotlandi; L W Fisher; N Zini; M P Colombo; M Campanacci; N M Maraldi; S Olivari; N Baldini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Human pathology     Volume:  23     ISSN:  0046-8177     ISO Abbreviation:  Hum. Pathol.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-01-27     Completed Date:  1993-01-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421547     Medline TA:  Hum Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1326-31     Citation Subset:  IM    
Laboratorio di Ricerca Oncologica, Istituti Ortopedici Rizzoli, Bologna, Italy.
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MeSH Terms
Blotting, Northern
Bone Neoplasms / chemistry*,  diagnosis*,  pathology
Microscopy, Electron
Microscopy, Immunoelectron
Osteoma, Osteoid / chemistry*,  diagnosis*,  pathology
Osteonectin / analysis*,  genetics,  metabolism
Osteosarcoma / chemistry*,  diagnosis*,  pathology
RNA, Neoplasm / analysis,  genetics
Tumor Cells, Cultured / chemistry,  metabolism,  pathology
Tumor Markers, Biological / analysis*
Reg. No./Substance:
0/Osteonectin; 0/RNA, Neoplasm; 0/Tumor Markers, Biological

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