| Evaluation and optimisation of preparative semi-automated electrophoresis systems for Illumina library preparation. | |
| | |
MedLine Citation:
|
PMID: 23147856 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Size selection can be a critical step in preparation of next-generation sequencing libraries. Traditional methods employing gel electrophoresis lack reproducibility, are labour intensive, do not scale well and employ hazardous interchelating dyes. In a high-throughput setting, solid-phase reversible immobilisation beads are commonly used for size-selection, but result in quite a broad fragment size range. We have evaluated and optimised the use of two semi-automated preparative DNA electrophoresis systems, the Caliper Labchip XT and the Sage Science Pippin Prep, for size selection of Illumina sequencing libraries. |
| | |
Authors:
|
Michael A Quail; Yong Gu; Harold Swerdlow; Matthew Mayho |
Related Documents
:
|
23683706 - Library construction and evaluation for site saturation mutagenesis. 1668606 - Cloning of the human erythropoietin receptor gene. 9156036 - Reduced expression and normal nucleotide sequence of androgen receptor gene coding and ... 8224916 - Sequence and organization of the human n-formyl peptide receptor-encoding gene. 1766876 - Detection of gene targeting by co-conversion of a single nucleotide change during repla... 8610176 - Identification and characterization of a ring zinc finger gene (c-rzf) expressed in chi... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-9-7 |
Journal Detail:
|
Title: Electrophoresis Volume: - ISSN: 1522-2683 ISO Abbreviation: Electrophoresis Publication Date: 2012 Sep |
Date Detail:
|
Created Date: 2012-11-13 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
|
Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Neural interfaces for the brain and spinal cord-restoring motor function.
Next Document: Intraocular Lens Power Measured by Partial Coherence Interferometry.