Document Detail


Evaluation and optimisation of preparative semi-automated electrophoresis systems for Illumina library preparation.
MedLine Citation:
PMID:  23147856     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Size selection can be a critical step in preparation of next-generation sequencing libraries. Traditional methods employing gel electrophoresis lack reproducibility, are labour intensive, do not scale well and employ hazardous interchelating dyes. In a high-throughput setting, solid-phase reversible immobilisation beads are commonly used for size-selection, but result in quite a broad fragment size range. We have evaluated and optimised the use of two semi-automated preparative DNA electrophoresis systems, the Caliper Labchip XT and the Sage Science Pippin Prep, for size selection of Illumina sequencing libraries.
Authors:
Michael A Quail; Yong Gu; Harold Swerdlow; Matthew Mayho
Related Documents :
23683706 - Library construction and evaluation for site saturation mutagenesis.
1668606 - Cloning of the human erythropoietin receptor gene.
24003196 - A cost-effective method for high-throughput construction of illumina sequencing libraries.
25055896 - Full-length characterization of transcribed genomic regions.
1766876 - Detection of gene targeting by co-conversion of a single nucleotide change during repla...
8610176 - Identification and characterization of a ring zinc finger gene (c-rzf) expressed in chi...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-7
Journal Detail:
Title:  Electrophoresis     Volume:  -     ISSN:  1522-2683     ISO Abbreviation:  Electrophoresis     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-11-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Neural interfaces for the brain and spinal cord-restoring motor function.
Next Document:  Intraocular Lens Power Measured by Partial Coherence Interferometry.