Document Detail


Evaluation of the number of goblet cells in crypts of the colonic mucosa with and without fecal transit.
MedLine Citation:
PMID:  22664521     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVE: To measure the thickness of the crypts and quantify the number of goblet cells of the colonic mucosa with and without intestinal transit, relating them to exclusion time. METHODS: Sixty Wistar rats were divided into three groups of 20 animals each according to the time of the final operation for the removal of the colon, in six, 12 or 18 weeks. In each group 15 animals underwent colonic exclusion by left colon proximal colostomy and distal mucous fistula, and five underwent only laparotomy (control). The colons with and without fecal stream were removed, processed and submitted to histological sections stained with hematoxylin-eosin. The height of the colonic crypts and the number of goblet cells were measured by computerized morphometry. We used the Student t test and Kruskal-Wallis test for comparison and analysis of variance, using a significance level of 5% (p <0.05). RESULTS: The height of the crypts decreased in segments without fecal stream (p =0.0001), reducing from six to 12 weeks of exclusion (p = 0.0003), stabilizing thereafter. The number of goblet cells in the crypts was smaller in segments without transit after 12 and 18 weeks (p = 0.0001), but increased as the time of exclusion progressed (p = 0.04) CONCLUSION: The exclusion of intestinal transit decreases the thickness of the colonic crypts and the number of goblet cells in the segments without transit. There is an increased number of goblet cells in the course of time exclusion.
Authors:
Rodrigo de Oliveira Mello; Camila Morais Gonçalves da Silva; Fábio Piovezan Fonte; Daniele Luchinitz Ferraz Silva; José Aires Pereira; Nelson Fontana Margarido; Carlos Augusto Real Martinez
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Revista do Colegio Brasileiro de Cirurgioes     Volume:  39     ISSN:  1809-4546     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-6-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7809515     Medline TA:  Rev Col Bras Cir     Country:  -    
Other Details:
Languages:  ENG; POR     Pagination:  139-145     Citation Subset:  -    
Affiliation:
Universidade São Francisco, Bragança Paulista, SP, BR.
Vernacular Title:
Avaliação do número de células caliciformes nas criptas da mucosa colônica com e sem trânsito intestinal.
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