| Evaluation of a novel short polyadenylation signal as an alternative to the SV40 polyadenylation signal. | |
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MedLine Citation:
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PMID: 16472858 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The soluble neuropilin-1 (sNRP-1) gene employs an extremely short dual function polyadenylation (pA) signal/stop codon that is efficient for termination of gene transcription and translation in vivo. However, the functionality and usefulness of this signal in regard to other genes is unknown. This quantitative study compares the levels of humanized Renilla green fluorescent protein (hrGFP) mRNA polyadenylated with either the sNRP-1 pA signal or the much larger, more widely used SV40 pA signal. We show that the overall starting copy number of hrGFP for equally loaded RNAs is equivalent between the two groups. Our data show little to no difference between levels of mRNA generated by the sNRP-1 polyadenylation signal and the SV40 polyadenylation signal despite a remarkable size difference in signal length. An extremely short polyadenylation signal could potentially alleviate gene insert size restrictions associated with cloning or with therapeutic vectors such as adeno-associated virus. |
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Authors:
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Trevor J McFarland; Yi Zhang; La-ongsri Atchaneeyaskul; Peter Francis; J Timothy Stout; Binoy Appukuttan |
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Publication Detail:
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Type: Journal Article Date: 2006-02-10 |
Journal Detail:
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Title: Plasmid Volume: 56 ISSN: 0147-619X ISO Abbreviation: Plasmid Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-05-22 Completed Date: 2006-07-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7802221 Medline TA: Plasmid Country: United States |
Other Details:
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Languages: eng Pagination: 62-7 Citation Subset: IM |
Affiliation:
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Casey Eye Institute, Oregon Health and Science University, 3375 SW Terwilliger Blvd, Portland, 97239-4197, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Cloning, Molecular Codon, Terminator Dependovirus / genetics Exons Genome Green Fluorescent Proteins / metabolism Humans Introns Molecular Sequence Data Neuropilin-1 / genetics* Oligopeptides / genetics* Polyadenylation* Protein Isoforms RNA, Messenger / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Codon, Terminator; 0/Oligopeptides; 0/Protein Isoforms; 0/RNA, Messenger; 0/SV40 nuclear localization peptide; 144713-63-3/Neuropilin-1; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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