Document Detail

Evaluation of a novel rapid detection system for yeasts and molds.
MedLine Citation:
PMID:  21212506     Owner:  NLM     Status:  MEDLINE    
A novel system, the NISSUI rapid detection system, has been developed to rapidly detect yeasts and molds in food. This system consists of a liquid medium containing resazurin as a redox indicator, a unique original micro-well dish containing 676 micro-wells, and a fluorescence-monitoring instrument with an incubator. To evaluate this system, orange juice, milk, and physiological saline solutions artificially inoculated with yeasts or molds were used as samples. Comparison of the new system used at 28ºC for 48 h with a spread-plate method using a potato-dextrose-agar plate at 25ºC for 120 h yielded a correlation coefficient (r) of 0.95. Our data reveal that the new method considerably shortens the time required for detection of yeasts and molds in food.
Kei Tominaga; Toshihiro Ii; Fumi Kashiwabara; Ryotaro Hara; Yoshihiko Uesaka; Hidemasa Kodaka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biocontrol science     Volume:  15     ISSN:  1342-4815     ISO Abbreviation:  Biocontrol Sci     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2011-01-07     Completed Date:  2011-01-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9712121     Medline TA:  Biocontrol Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  139-42     Citation Subset:  IM    
Research Department, Nissui Pharmaceutical Co. LTD., Ibaraki, Japan.
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MeSH Terms
Culture Media
Food Microbiology*
Fungi / isolation & purification*
Microbiological Techniques
Yeasts / isolation & purification*
Reg. No./Substance:
0/Culture Media

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