Document Detail


Evaluation of a novel hybrid bioartificial liver based on a multi-layer flat-plate bioreactor.
MedLine Citation:
PMID:  22851870     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To evaluate the efficacy and safety of a hybrid bioartificial liver (HBAL) system in the treatment of acute liver failure.
METHODS: Canine models with acute liver failure were introduced with intravenous administration of D-galactosamine. The animals were divided into: the HBAL treatment group (n = 8), in which the canines received a 3-h treatment of HBAL; the bioartificial liver (BAL) treatment group (n = 8), in which the canines received a 3-h treatment of BAL; the non-bioartificial liver (NBAL) treatment group (n = 8), in which the canines received a 3-h treatment of NBAL; the control group (n = 8), in which the canines received no additional treatment. Biochemical parameters and survival time were determined. Levels of xenoantibodies, RNA of porcine endogenous retrovirus (PERV) and reverse transcriptase (RT) activity in the plasma were detected.
RESULTS: Biochemical parameters were significantly decreased in all treatment groups. The TBIL level in the HBAL group was lower than that in other groups (2.19 ± 0.55 μmol/L vs 24.2 ± 6.45 μmol/L, 12.47 ± 3.62 μmol/L, 3.77 ± 1.83 μmol/L, P < 0.05). The prothrombin time (PT) in the BAL and HBAL groups was significantly shorter than the NBAL and control groups (18.47 ± 4.41 s, 15.5 ± 1.56 s vs 28.67 ± 5.71 s, 21.71 ± 3.4 s, P < 0.05), and the PT in the HBAL group was shortest of all the groups. The albumin in the BAL and HBAL groups significantly increased and a significantly higher level was observed in the HBAL group compared with the BAL group (27.7 ± 1.7 g/L vs 25.24 ± 1.93 g/L). In the HBAL group, the ammonia levels significantly decreased from 54.37 ± 6.86 to 37.75 ± 6.09 after treatment (P < 0.05); there were significant difference in ammonia levels between other the groups (P < 0.05). The levels of antibodies were similar before and after treatment. The PERV RNA and the RT activity in the canine plasma were all negative.
CONCLUSION: The HBAL showed great efficiency and safety in the treatment of acute liver failure.
Authors:
Xiao-Lei Shi; Yue Zhang; Xue-Hui Chu; Bing Han; Jin-Yang Gu; Jiang-Qiang Xiao; Jia-Jun Tan; Zhong-Ze Gu; Hao-Zhen Ren; Xian-Wen Yuan; Yi-Tao Ding
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  18     ISSN:  2219-2840     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-08-01     Completed Date:  2013-01-10     Revised Date:  2014-05-20    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  3752-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Heterophile / chemistry
Bioreactors*
Coculture Techniques
Dogs
Endogenous Retroviruses / metabolism
Galactosamine / metabolism
Liver Failure, Acute / therapy*
Liver, Artificial*
Organ Culture Techniques / methods
Prothrombin Time
RNA / metabolism
RNA-Directed DNA Polymerase / metabolism
Swine
Time Factors
Chemical
Reg. No./Substance:
0/Antibodies, Heterophile; 63231-63-0/RNA; 7535-00-4/Galactosamine; EC 2.7.7.49/RNA-Directed DNA Polymerase
Comments/Corrections

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