| Evaluation of new monoclonal antibody-based latex agglutination test for detection of cryptococcal polysaccharide antigen in serum and cerebrospinal fluid. | |
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MedLine Citation:
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PMID: 7814566 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We evaluated the performance of CRYPTO-LEX (Trinity Laboratories, Inc., Raleigh, N. C.), a new mouse immunoglobulin M monoclonal antibody latex agglutination reagent which reacts with the capsular polysaccharide of the four serogroups of Cryptococcus neoformans. This test was compared with CALAS (Meridian Diagnostics, Cincinnati, Ohio) for the ability to detect cryptococcal antigen in serum and cerebrospinal fluid (CSF). A total of 580 clinical specimens (327 serum and 253 CSF samples), primarily from human immunodeficiency virus-infected patients, were tested in this study. Sixty-seven specimens (44 serum and 23 CSF samples) were positive for cryptococcal antigen with both tests, and 511 (282 serum and 229 CSF samples) were negative. The two latex reagents agreed for 326 of 327 serum specimens (44 positives and 282 negatives). One serum specimen with a titer of 1:2 was CALAS positive but CRYPTO-LEX negative. The titer correlation coefficient for the two tests was 0.884 when two highly discordant serum specimens were eliminated from analysis of the data. The two latex tests agreed for 252 of 253 CSF specimens (23 positives and 229 negatives). One specimen with a titer of 1:2 was positive with CALAS and negative by CRYPTO-LEX. The correlation coefficient of the two tests for CSF titers was 0.886. The sensitivity and specificity of CRYPTO-LEX were 97 and 100%, respectively, with a 99.6% correlation with CALAS. These data show that the performance of CRYPTO-LEX is comparable to that of CALAS for detection of cryptococcal antigen in serum and CSF. |
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Authors:
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D L Kiska; D R Orkiszewski; D Howell; P H Gilligan |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 32 ISSN: 0095-1137 ISO Abbreviation: J. Clin. Microbiol. Publication Date: 1994 Sep |
Date Detail:
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Created Date: 1995-02-06 Completed Date: 1995-02-06 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2309-11 Citation Subset: IM; X |
Affiliation:
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Clinical Microbiology Laboratory, University of North Carolina Hospitals, Chapel Hill 27514. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Fungal / immunology* Antibodies, Monoclonal / immunology* Antigens, Fungal / analysis*, blood, cerebrospinal fluid Cryptococcosis / blood, cerebrospinal fluid, diagnosis* Evaluation Studies as Topic Humans Immunoglobulin M / immunology Latex Fixation Tests* Mice Polysaccharides / analysis*, blood, cerebrospinal fluid Predictive Value of Tests Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Fungal; 0/Antibodies, Monoclonal; 0/Antigens, Fungal; 0/Immunoglobulin M; 0/Polysaccharides; 0/cryptococcal polysaccharide |
| Comments/Corrections | |
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