Document Detail

Evaluation of neuronal cell death after a new global ischemia model in infant mice.
MedLine Citation:
PMID:  14753414     Owner:  NLM     Status:  MEDLINE    
For the first time we set up a new model for global ischemia in the infant mice, and time-dependent changes of the blood-brain barrier (BBB) disruption and neuronal cell death were investigated in detail. Infant C57/B16 mice (postnatal 13 days) were anesthetized with inhalation of sevoflurane in N2O/O2 (70/30%) and were subjected to global ischemia by bilateral common carotid artery occlusion (CCAO) for 25 minutes. Disruption of BBB was noted at 4 hours and increased up to 24 hours after the injection of 2% Evan's Blue in the transient CCAO (tCCAO) model. Evaluation of neuronal cell death was determined with toluidine blue staining. Morphological changes of neurons after tCCAO were clearly observed in the hippocampal CA1 region but were slightly detected in the CA3 region. However, there were no morphological changes in the hippocampal dentate gyrus, the neocortex, the striatum and the hypothalamus. The number of survival neurons in the CA1 was significantly decreased at 2 days and sustained up to 4 days after tCCAO. These data indicate that this method is very useful to induce selective vulnerability in mouse hippocampus, and it provides a reliable ischemic model in infant mice.
H Ohtaki; S Mori; T Nakamachi; K Dohi; L Yin; S Endo; Y Okada; S Shioda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta neurochirurgica. Supplement     Volume:  86     ISSN:  0065-1419     ISO Abbreviation:  Acta Neurochir. Suppl.     Publication Date:  2003  
Date Detail:
Created Date:  2004-02-02     Completed Date:  2004-03-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100962752     Medline TA:  Acta Neurochir Suppl     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  97-100     Citation Subset:  IM    
Department of Anatomy, Showa University School of Medicine, Tokyo, Japan.
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MeSH Terms
Animals, Newborn
Arterial Occlusive Diseases / complications
Blood-Brain Barrier
Brain Ischemia / complications,  etiology,  pathology,  physiopathology*
Carotid Artery, Common
Cell Death
Mice, Inbred C57BL
Nerve Degeneration / etiology,  pathology

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