| Evaluation of neonatal neutrophil function using the combined immunobead/NBT dye reduction assay. | |
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MedLine Citation:
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PMID: 7069780 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An improved assay for the simultaneous assessment of phagocytic uptake (via Immunobeads) and metabolic integrity (via NBT dye reduction) was used to evaluate the neutrophil function in neonates, one year olds, and adults. The unique advantage of this assay is that it evaluates both phagocytic and killing function simultaneously which appears to make the assay more reliable in the detection of neutrophil dysfunction. This assay allowed us to establish a subtle but significant neutrophil dysfunction in full term unstressed neonates which proved to be more pronounced in both the stressed and the preterm neonates. The one year olds, however, were found to have neutrophil function comparable to that of an adult. |
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Authors:
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A B Loren; J C Maggi; J P Scott; C L Anderson; Y Niwa; M M Yokoyama |
Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Journal of clinical & laboratory immunology Volume: 7 ISSN: 0141-2760 ISO Abbreviation: J Clin Lab Immunol Publication Date: 1982 Jan |
Date Detail:
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Created Date: 1982-06-24 Completed Date: 1982-06-24 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7808987 Medline TA: J Clin Lab Immunol Country: ITALY |
Other Details:
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Languages: eng Pagination: 71-4 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Age Factors Female Humans Immunoassay Infant Infant, Newborn* Infant, Premature Neutrophils / physiology* Nitroblue Tetrazolium / metabolism* Oxidation-Reduction Phagocytosis* Pregnancy Stress, Physiological / blood Tetrazolium Salts / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Tetrazolium Salts; 298-83-9/Nitroblue Tetrazolium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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