Document Detail


Evaluation of neonatal neutrophil function using the combined immunobead/NBT dye reduction assay.
MedLine Citation:
PMID:  7069780     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An improved assay for the simultaneous assessment of phagocytic uptake (via Immunobeads) and metabolic integrity (via NBT dye reduction) was used to evaluate the neutrophil function in neonates, one year olds, and adults. The unique advantage of this assay is that it evaluates both phagocytic and killing function simultaneously which appears to make the assay more reliable in the detection of neutrophil dysfunction. This assay allowed us to establish a subtle but significant neutrophil dysfunction in full term unstressed neonates which proved to be more pronounced in both the stressed and the preterm neonates. The one year olds, however, were found to have neutrophil function comparable to that of an adult.
Authors:
A B Loren; J C Maggi; J P Scott; C L Anderson; Y Niwa; M M Yokoyama
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of clinical & laboratory immunology     Volume:  7     ISSN:  0141-2760     ISO Abbreviation:  J Clin Lab Immunol     Publication Date:  1982 Jan 
Date Detail:
Created Date:  1982-06-24     Completed Date:  1982-06-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7808987     Medline TA:  J Clin Lab Immunol     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  71-4     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Age Factors
Female
Humans
Immunoassay
Infant
Infant, Newborn*
Infant, Premature
Neutrophils / physiology*
Nitroblue Tetrazolium / metabolism*
Oxidation-Reduction
Phagocytosis*
Pregnancy
Stress, Physiological / blood
Tetrazolium Salts / metabolism*
Chemical
Reg. No./Substance:
0/Tetrazolium Salts; 298-83-9/Nitroblue Tetrazolium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Competition for immune complexes by red cells in human blood.
Next Document:  Defects of leukocyte locomotion in multiple myeloma.