Document Detail


Evaluation of mutagenic effects of formocresol: detection of DNA-protein cross-links and micronucleus in mouse bone marrow.
MedLine Citation:
PMID:  18206398     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The genotoxic potential of formocresol was assessed by comet assay on human peripheral blood lymphocytes and in vivo micronucleus in mice. STUDY DESIGN: Peripheral blood lymphocytes, obtained from healthy donors, were exposed directly with different dilutions of formocresol for 45 minutes at 37 degrees C. To verify the possibility of formocresol to induce DNA-protein cross-links, treated lymphocytes were incubated with proteinase K. Micronucleus test was performed on male Swiss mice treated with several dilutions of formocresol by single intraperitoneal injection. After treatment, bone marrow was sampled 24 and 48 hours after formocresol administration. RESULTS: Formocresol did not produce detectable DNA damage as evaluated by comet assay. However, after proteinase K exposure, a dose-dependent increase of DNA migration was observed. Formocresol induced a significant increase in micronucleus frequencies at the highest dilution only at 24 hours after administration. CONCLUSION: Formocresol induced DNA-protein cross-links and an increased frequency of micronucleus.
Authors:
Maria Emília Santos Pereira Ramos; Bruno Coêlho Cavalcanti; Letícia Veras Costa Lotufo; Manoel Odorico de Moraes; Eneida de Moraes Marcílio Cerqueira; Cláudia Pessoa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics     Volume:  105     ISSN:  1528-395X     ISO Abbreviation:  Oral Surg Oral Med Oral Pathol Oral Radiol Endod     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-18     Completed Date:  2008-04-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508562     Medline TA:  Oral Surg Oral Med Oral Pathol Oral Radiol Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  398-404     Citation Subset:  D; IM    
Affiliation:
Department of Health, Feira de Santana State University, Feira de Santana, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Marrow Cells / drug effects
Cells, Cultured
Comet Assay
Cross-Linking Reagents
DNA Adducts*
Dental Materials / toxicity*
Formocresols / toxicity*
Humans
Lymphocytes / drug effects
Male
Mice
Micronucleus Tests
Mutagenesis*
Chemical
Reg. No./Substance:
0/Cross-Linking Reagents; 0/DNA Adducts; 0/Dental Materials; 0/Formocresols; 37203-87-5/formocresol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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