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Evaluation of multiplex Polymerase chain reaction utilising multiple targets in Mycobacterium tuberculosis direct test negative but culture positive cases: A potential method for enhancing the diagnosis of tuberculosis.
MedLine Citation:
PMID:  24064644     Owner:  NLM     Status:  In-Data-Review    
Purpose: To evaluate multiplex Polymerase Chain Reaction (MPCR) utilising multiple targets (IS6110, Protein b [Pab] and MPB64 genes) in Mycobacterium tuberculosis Direct Test (MTD) negative but culture positive cases and comparison of MPCR with Real-Time polymerase chain reaction (RT-PCR) for diagnosis of tuberculosis. Materials and Methods: MPCR was carried out on 28 culture positive sputum samples. Out of 28 culture positive samples, 17 were originally reported, as MTD test negative and 11 were MTD test positive, respectively. The results of MPCR were compared with RT-PCR. To check the specificity of the tests, MPCR and RT-PCR were also evaluated with 16 non-tuberculous mycobacterial (NTM) isolates. Results: Out of 28 culture positive sputum samples, MPCR was positive in all 28/28 samples, whereas RT-PCR was positive in 27/28 samples and MTD test was originally tested positive in six sputum samples and on repeating MTD testing, five more sputum samples were positive and thus total number of MTD positive were 11/28 sputum samples, respectively. All the tests were negative on evaluation with all the 16 NTMs, thus giving specificity of 100% to all the tests; sensitivity of MPCR, RT-PCR and MTD tests were 100%, 96.42% and 39.28%, respectively, in these specifically selected samples. Conclusions: MPCR may be an important tool in the rapid diagnosis of tuberculosis especially in disease endemic, resource limited countries.
K Sharma; D Ashkin; P Fiorella; D Willis; S Dean; A Sharma; K K Singh; Y Lee; M Pedrosa; G Singh; M Sharma; S Laal
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Indian journal of medical microbiology     Volume:  31     ISSN:  1998-3646     ISO Abbreviation:  Indian J Med Microbiol     Publication Date:    2013 Oct-Dec
Date Detail:
Created Date:  2013-09-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8700903     Medline TA:  Indian J Med Microbiol     Country:  India    
Other Details:
Languages:  eng     Pagination:  370-3     Citation Subset:  IM    
Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
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