Document Detail


Evaluation of a multicolor, single-tube technique to enumerate lymphocyte subpopulations.
MedLine Citation:
PMID:  18448621     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To evaluate the fully automated FACSCanto software, we compared lymphocyte subpopulation counts obtained using three-color FACSCalibur-CELLQuest and six-color FACSCanto-FACSCanto software techniques. High correlations were observed between data obtained with these techniques. Our study indicated that FACSCanto clinical software is accurate and sensitive in single-platform lymphocyte immunophenotyping.
Authors:
F Colombo; A Cattaneo; R Lopa; P Portararo; P Rebulla; L Porretti
Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2008-04-30
Journal Detail:
Title:  Clinical and vaccine immunology : CVI     Volume:  15     ISSN:  1556-679X     ISO Abbreviation:  Clin. Vaccine Immunol.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-11     Completed Date:  2008-09-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  101252125     Medline TA:  Clin Vaccine Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1124-7     Citation Subset:  IM    
Affiliation:
Cellular Therapy and Cryobiology, Regenerative Medicine Department, Centre of Transfusion Medicine, IRCCS Fondazione Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Milan, Italy. labcitofl@policlinico.mi.it
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antigens, CD / immunology
Flow Cytometry / methods*
Humans
Immunophenotyping / methods*
Lymphocyte Subsets / immunology*
Sensitivity and Specificity
Software
Chemical
Reg. No./Substance:
0/Antigens, CD
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effects of delays in peripheral blood processing, including cryopreservation, on detection of CD31 e...
Next Document:  Evaluation of seven tests for diagnosis of human brucellosis in an area where the disease is endemic...