Document Detail

Evaluation and modelling the utility of SCCO(2) to support efficient lipase mediated esterification.
MedLine Citation:
PMID:  22112570     Owner:  NLM     Status:  In-Data-Review    
Supercritical fluids offer environmental advantages over chemical solvents, while providing enhanced separation and chemical selectivity. The use of supercritical fluids for the recovery of products from biomass and the transformation of selected molecules (to add value) was studied. Free fatty acids were bio-catalytically transformed to fatty acid esters using lipase within a supercritical fluid environment. A central composite rotatable design was used to evaluate the influence of operating conditions on the enzymatic esterification process and a response surface equation was optimized to identify the most favourable process conditions for maximum free fatty acid conversion. Based on the model equation the process conditions under which it was predicted a yield of 100% esters could be obtained were: pressure 200bar, temperature 60°C, ethanol concentration 2.0M, enzyme concentration 11wt.% and time 60min. Experiments conducted under these conditions gave an ester yield of 94.3% (close to predicted results). The activity per unit mass of biocatalyst was found to be 1585μmol/min/g(cat). The results support the use of supercritical fluids for process integration.
M N Baig; R C D Santos; C Zetzl; J King; D Pioch; S Bowra
Publication Detail:
Type:  Journal Article     Date:  2011-07-19
Journal Detail:
Title:  Enzyme and microbial technology     Volume:  49     ISSN:  0141-0229     ISO Abbreviation:  Enzyme Microb. Technol.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-11-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8003761     Medline TA:  Enzyme Microb Technol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  420-6     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
School of Chemical Engineering, University of Birmingham, Edgbaston, B15 2TT Birmingham, United Kingdom.
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