Document Detail


Evaluation of the mitochondrial respiratory chain and oxidative phosphorylation system using yeast models of OXPHOS deficiencies.
MedLine Citation:
PMID:  19806592     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The oxidative phosphorylation (OXPHOS) system consists of five multimeric complexes embedded in the mitochondrial inner membrane. They work in concert to drive the aerobic synthesis of ATP. Mitochondrial and nuclear DNA mutations affecting the accumulation and function of these enzymes are the most common cause of mitochondrial diseases and have also been associated with neurodegeneration and aging. Several approaches for the assessment of the OXPHOS system enzymes have been developed. Based on the methods described elsewhere, this unit describes the creation and study of yeast models of mitochondrial OXPHOS deficiencies.
Authors:
Flavia Fontanesi; Francisca Diaz; Antoni Barrientos
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]     Volume:  Chapter 19     ISSN:  1934-8258     ISO Abbreviation:  Curr Protoc Hum Genet     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-10-06     Completed Date:  2009-10-14     Revised Date:  2011-09-26    
Medline Journal Info:
Nlm Unique ID:  101287858     Medline TA:  Curr Protoc Hum Genet     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit19.5     Citation Subset:  IM    
Affiliation:
University of Miami Miller School of Medicine, Miami, Florida, USA.
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MeSH Terms
Descriptor/Qualifier:
Humans
Mitochondria / chemistry*,  metabolism
Models, Biological
Mutation
Oxidative Phosphorylation*
Saccharomyces cerevisiae / chemistry*,  genetics,  metabolism
Saccharomyces cerevisiae Proteins / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
R01 GM071775-01A2/GM/NIGMS NIH HHS; R01 GM071775A/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Saccharomyces cerevisiae Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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