| Evaluation of methods for the identification of Salmonella enterica serotype Typhimurium DT104 from poultry environmental samples. | |
| | |
MedLine Citation:
|
PMID: 15177906 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
An increase in the prevalence of Salmonella enterica serotype Typhimurium DT104 has been reported worldwide. This study examined the prevalence of this microorganism in poultry environmental samples from commercial layer flocks and pullet environments as well as the sensitivity and specificity of a PCR-based method, and multiple antibiotic resistance profile of Salmonella serogroup B isolates in relation to the serotype and phagetype reference method for the identification of Salmonella Typhimurium DT104. A total of 435 Salmonella isolates were obtained from poultry house environmental samples tested during a 20-month period representing a prevalence of 5.5%. Of these, 313 (72%) isolates were identified as Salmonella serogroup B isolates. These isolates were tested by a PCR-based assay, and for resistance to five antibiotics: ampicillin, chloramphenicol, streptomycin, sulfonamides, and tetracycline (ACSSuT) for the rapid identification of Salmonella Typhimurium DT104. Upon comparing the antibiotic resistance and PCR results with serotype and phage type data, the sensitivity and specificity for the identification of Salmonella Typhimurium DT104 of both methods were found to be 100%, and 99.6%, respectively. Both methods can be completed within 24 h after obtaining an isolate, while serotyping and phagetyping required more than 5 days to complete. |
| | |
Authors:
|
Carlos G Leon-Velarde; Hugh Y Cai; Carolyn Larkin; Patricia Bell-Rogers; R William C Stevens; Joseph A Odumeru |
Related Documents
:
|
19819256 - Ten years experience of salmonella infections in cambridge, uk. 16082406 - Epidemiology of typhoid and paratyphoid fever in kathmandu: two years study and trends ... 18991546 - Characterization of multidrug-resistant salmonella enterica serovar heidelberg isolated... 3957766 - Emergence of antibiotic-resistant salmonella agona in horses in kentucky. 7509786 - Hispidospermidin, a novel phospholipase c inhibitor produced by chaetosphaeronema hispi... 9593036 - Enteroaggregative escherichia coli strains as a cause of traveler's diarrhea: a case-co... |
Publication Detail:
|
Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of microbiological methods Volume: 58 ISSN: 0167-7012 ISO Abbreviation: J. Microbiol. Methods Publication Date: 2004 Jul |
Date Detail:
|
Created Date: 2004-06-04 Completed Date: 2004-07-30 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8306883 Medline TA: J Microbiol Methods Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 79-86 Citation Subset: IM |
Copyright Information:
|
Copyright 2004 Elsevier B.V. |
Affiliation:
|
Laboratory Services Division, University of Guelph, 95 Stone Rd. West, Guelph, ON, Canada N1H 8J7. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Bacteriophage Typing / veterinary DNA, Bacterial / chemistry, genetics Drug Resistance, Multiple, Bacterial Female Housing, Animal Ontario / epidemiology Polymerase Chain Reaction / veterinary Poultry Poultry Diseases / epidemiology, microbiology* Prevalence Salmonella Infections, Animal / epidemiology, microbiology* Salmonella typhimurium / genetics, isolation & purification* Sensitivity and Specificity Serotyping / veterinary |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A protocol for quantifying the birth, death and emigration rates of produce-associated bacteria, ill...
Next Document: Methods evaluating vanadium tolerance in bacteria isolated from crude oil contaminated land.