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Evaluation of a method allowing preservation of fresh lymph nodes for flow cytometric immunophenotyping.
MedLine Citation:
PMID:  22499436     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: Flow cytometric immunophenotyping (FCI) of lymph nodes (LN) requires fresh unfixed tissue, with analysis being carried out within few hours post surgery. This study evaluated a novel method for fresh LN preservation, in order to allow histomorphology-based FCI. METHODS: This study was carried out prospectively on 30 LN with suspected involvement by Haematolymphoid Neoplasms (HLN). FCI was performed on each fresh and post cryopreserved LN cell suspension. Percentage positivities (PP) and Mean Fluorescent Intensities (MFI) were calculated on both preparations for a combination of T and B-lymphoid antigens together with viability. RESULTS: The cryopreservation method applied within this study did not affect significantly PP and had minor impact on MFI of the tested antigens. Overall there was minimal decrease in PP and MFI on the cryopreserved cells when compared to fresh cells, for most antigens with only a mild increase in apoptotic cells. However these changes were not diagnostically significant, since both reactive processes and HLN present within analysed LN could be identified and differentiated. Viability was more than 75% for all cryopreserved LN composed of haematolymphoid cells. CONCLUSIONS: The method presented in this study confers the possibility of storing fresh LN biopsies for later FCI, thus allowing a morphology-based immunophenotypic approach. This would allow a more sensitive, specific and cost-effective management of LN specimens, whilst maintaining the important benefits provided by FCI. © 2012 International Clinical Cytometry Society.
Authors:
Patricia Brincat; James Degaetano; Craig Donaldson
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-12
Journal Detail:
Title:  Cytometry. Part B, Clinical cytometry     Volume:  -     ISSN:  1552-4957     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101235690     Medline TA:  Cytometry B Clin Cytom     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 International Clinical Cytometry Society.
Affiliation:
MSc. Haematology (University of the West of England), Haematology Laboratory Pathology Department, Mater Dei Hospital, Malta. patricia.farrugia@gov.mt.
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