Document Detail


Evaluation of a low cost cryopreservation system on the biology of human amniotic fluid-derived mesenchymal stromal cells.
MedLine Citation:
PMID:  22280955     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: Human amniotic-derived mesenchymal stromal cells (hAMSC) are a novel population of multipotent stem cells that have been shown to have great potential for use in regenerative medicine. However, procedures to store and preserve hAMSC for future clinical applications have not been explored extensively. METHODS: In this study, we analyzed the influence of cryopreservation, using a protocol based on freezing rate of 1°C/min, 10% dimethyl sulfoxide as cryoprotectant and a thawing rate >100°C/min, on hAMSC morphology, proliferation rates, viability, cell cycle, karyotype, immune phenotype and multilineage differentiation potential. RESULTS: This study found that this cryopreservation protocol does not affect the biological properties of hAMSC. DISCUSSION: This shows that this protocol is a viable system for banking hAMSC, with the associated advantages that has a low cost in terms of expense, time and personnel involved and is easy to implement.
Authors:
Jose Maria Miranda-Sayago; Nieves Fernandez-Arcas; Carmen Benito; Armando Reyes-Engel; Jose Ramon Herrero; Antonio Alonso
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-20
Journal Detail:
Title:  Cryobiology     Volume:  -     ISSN:  1090-2392     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Inc.
Affiliation:
Department of Immunology, Carlos Haya Regional University Hospital, Malaga 29010, Spain; Department of Biochemistry, Molecular Biology and Immunology, Faculty of Medicine, University of Malaga, Malaga 29071, Spain.
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