Document Detail


Evaluation of live-attenuated Salmonella vaccines expressing Campylobacter antigens for control of C. jejuni in poultry.
MedLine Citation:
PMID:  19853682     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Campylobacter jejuni is a zoonotic bacterial pathogen of worldwide importance. It is estimated that 460,000 human infections occur in the United Kingdom per annum and these involve acute enteritis and may be complicated by severe systemic sequelae. Such infections are frequently associated with the consumption of contaminated poultry meat and strategies to control C. jejuni in poultry are expected to limit pathogen entry into the food chain and the incidence of human disease. Toward this aim, a total of 840 Light Sussex chickens were used to evaluate a Salmonella enterica serovar Typhimurium DeltaaroA vaccine expressing the C. jejuni amino acid binding protein CjaA as a plasmid-borne fusion to the C-terminus of fragment C of tetanus toxin. Chickens were given the vaccine at 1-day-old and two weeks later by oral gavage, then challenged after a further two weeks with C. jejuni. Across six biological replicates, statistically significant reductions in caecal C. jejuni of c. 1.4log(10) colony-forming units/g were observed at three and four weeks post-challenge relative to age-matched unvaccinated birds. Protection was associated with the induction of CjaA-specific serum IgY and biliary IgA. Protection was not observed using a vaccine strain containing the empty plasmid. Vaccination with recombinant CjaA subcutaneously at the same intervals significantly reduced the caecal load of C. jejuni at three and four weeks post-challenge. Taken together these data imply that responses directed against CjaA, rather than competitive or cross-protective effects mediated by the carrier, confer protection. The impact of varying parameters on the efficacy of the S. Typhimurium DeltaaroA vaccine expressing TetC-CjaA was also tested. Delaying the age at primary vaccination had little impact on protection or humoral responses to CjaA. The use of the parent strain as carrier or changing the attenuating mutation of the carrier to DeltaspaS or DeltassaU enhanced the protective effect, consistent with increased invasion and persistence of the vaccine strains relative to the DeltaaroA mutant. Expression in the DeltaaroA strain of a TetC fusion to Peb1A, but not TetC fusions to GlnH or ChuA, elicited protection against intestinal colonisation by C. jejuni that was comparable to that observed with the TetC-CjaA fusion. Our data are rendered highly relevant by use of the target host in large numbers and support the potential of CjaA- and Peb1A-based vaccines for control of C. jejuni in poultry.
Authors:
Anthony M Buckley; Jinhong Wang; Debra L Hudson; Andrew J Grant; Michael A Jones; Duncan J Maskell; Mark P Stevens
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-22
Journal Detail:
Title:  Vaccine     Volume:  28     ISSN:  1873-2518     ISO Abbreviation:  Vaccine     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-02-17     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  8406899     Medline TA:  Vaccine     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1094-105     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
ATP-Binding Cassette Transporters / genetics,  immunology*
Administration, Oral
Amino Acid Transport Systems, Neutral / genetics,  immunology*
Animals
Antibodies, Bacterial / analysis
Bacterial Vaccines / genetics,  immunology*
Campylobacter Infections / prevention & control,  veterinary*
Campylobacter jejuni / genetics,  immunology*
Cecum / microbiology
Chickens
Colony Count, Microbial
Gastrointestinal Tract / immunology
Genetic Vectors*
Great Britain
Humans
Poultry Diseases / immunology,  prevention & control*
Recombinant Fusion Proteins / genetics,  immunology
Salmonella Vaccines / genetics,  immunology
Salmonella typhimurium / genetics*
Tetanus Toxin / genetics
Vaccines, Attenuated / genetics,  immunology
Vaccines, Synthetic / genetics,  immunology
Grant Support
ID/Acronym/Agency:
BB/D00019X/1//Biotechnology and Biological Sciences Research Council; BB/D000866/1//Biotechnology and Biological Sciences Research Council; G0801161//Medical Research Council
Chemical
Reg. No./Substance:
0/Amino Acid Transport Systems, Neutral; 0/Antibodies, Bacterial; 0/Bacterial Vaccines; 0/CjaA protein, Campylobacter jejuni; 0/Recombinant Fusion Proteins; 0/Salmonella Vaccines; 0/Tetanus Toxin; 0/Vaccines, Attenuated; 0/Vaccines, Synthetic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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