| Evaluation of laser-speckle contrast image analysis techniques in the cortical microcirculation of piglets. | |
| | |
MedLine Citation:
|
PMID: 22306444 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
A new laser speckle-contrast analysis (LASCA) technique based on multi-exposure imaging was employed to simultaneously study pial arteriolar responses with cerebrocortical perfusion changes to various vasodilator (5-10% CO(2) ventilation, bradykinin (1-10μM), N-methyl-d-aspartate (100μM)) vasoconstrictor (10-100μM noradrenaline, 1M KCl), or neutral (2.1% H(2) ventilation) stimuli as well as to asphyxia in the newborn piglet. Anesthetized, ventilated animals (n=20) were fitted with closed cranial windows. Multiple exposure laser-speckle image series (1-100ms) were obtained using a near infrared diode laser (λ=808nm). The autocorrelation decay time (τ) of speckle fluctuations was determined over pial arterioles and parenchymal areas to express 1/τ being proportional to blood flow velocity by two different LASCA techniques: our novel multi-exposure or a single exposure (2 and 20ms) approach. 1/τ values yielded by different LASCA techniques were not significantly different at most points. LASCA easily detected both increases and decreases in cortical blood flow (CoBF). Cortical 1/τ changes to hypercapnia closely matched quantitative CoBF data determined previously, and were also in accordance with increases of pial arteriolar blood flow, calculated from arteriolar flow velocity and cross sectional area changes. In summary, LASCA emerges as an appealing method to simultaneously study microvascular reactivity and cortical perfusion changes in the piglet. |
| | |
Authors:
|
Ferenc Domoki; Dániel Zölei; Orsolya Oláh; Valéria Tóth-Szűki; Béla Hopp; Ferenc Bari; Tomi Smausz |
Related Documents
:
|
236664 - Distribution of h+ and hco3 minus between csf and blood during respiratory acidosis in ... 16520544 - The effect of 7.2% hypertonic saline solution on the duration of sodium gradient betwee... 2309924 - Effect of water or saline intake on heat-induced limb vasodilation in dehydrated baboons. 7341814 - Transvascular fluid shift after blood volume modification in relation to compliances of... 21517584 - Nonlinear sand bedform dynamics in a viscous flow. 6117544 - Effects of norepinephrine and alpha-block on o2 uptake and blood flow in dog hindlimb. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-28 |
Journal Detail:
|
Title: Microvascular research Volume: - ISSN: 1095-9319 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-2-6 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0165035 Medline TA: Microvasc Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012. Published by Elsevier Inc. |
Affiliation:
|
Department of Physiology, Faculty of Medicine, University of Szeged, Szeged, Hungary. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Assessment of administrative data for evaluating the shifting acquisition of Clostridium difficile i...
Next Document: Ion beam induced amorphization and bond breaking in Zn(2)SiO(4):Eu(3+) nanocrystalline phosphor.