Document Detail


Evaluation of inhibitors of fatty acid oxidation in rat myocytes.
MedLine Citation:
PMID:  3689454     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of 4-bromocrotonic acid, 2-bromopalmitic acid, 3-mercaptopropionic acid, 4-pentenoic acid, and 2-tetradecylglycidic acid on the oxidations of palmitate, octanoate, and pyruvate in adult rat myocytes were studied. Since all of these compounds inhibit the oxidation of palmitate but not of pyruvate, they are specific inhibitors of fatty acid oxidation. Fifty percent inhibition of palmitate oxidation was obtained when myocytes were preincubated for 10 min with one of the following: 0.1 microM 2-tetradecylglycidic acid, 60 microM 4-bromocrotonic acid, 60 microM 2-bromopalmitic acid, 100 microM 3-mercaptoproprionic acid, or 100 microM 4-pentenoic acid. Removal of the inhibitors from the medium after preincubation relieved the inhibition caused by 3-mercaptopropionic acid but did not reverse the effects of the other inhibitors. This study leads to the conclusion that 2-tetradecylglycidic acid is the compound of choice for inhibiting the mitochondrial uptake of fatty acids and thereby their oxidation, whereas 4-bromocrotonic acid is the best irreversible inhibitor of the mitochondrial beta-oxidation cycle.
Authors:
S A el-Aleem; H Schulz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical pharmacology     Volume:  36     ISSN:  0006-2952     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  1987 Dec 
Date Detail:
Created Date:  1988-01-21     Completed Date:  1988-01-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  4307-12     Citation Subset:  IM    
Affiliation:
Department of Chemistry, City College of the City University of New York, NY 10031.
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MeSH Terms
Descriptor/Qualifier:
3-Mercaptopropionic Acid / pharmacology
Animals
Cells, Cultured
Crotonic Acids / pharmacology
Epoxy Compounds / pharmacology
Fatty Acids / metabolism*,  pharmacology
Fatty Acids, Monounsaturated / pharmacology
Myocardium / cytology,  metabolism*
Oxidation-Reduction
Palmitates / pharmacology
Rats
Grant Support
ID/Acronym/Agency:
HL 18089/HL/NHLBI NIH HHS; HL 30847/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Crotonic Acids; 0/Epoxy Compounds; 0/Fatty Acids; 0/Fatty Acids, Monounsaturated; 0/Palmitates; 107-96-0/3-Mercaptopropionic Acid; 13991-36-1/4-bromocrotonic acid; 18263-25-7/2-bromopalmitate; 591-80-0/4-pentenoic acid; 68170-97-8/2-tetradecylglycidic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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