Document Detail


Evaluation of the inclusion of a mixture of organic acids or lactulose into the feed of pigs experimentally challenged with Salmonella Typhimurium.
MedLine Citation:
PMID:  19914785     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A mixture of organic acids and lactulose for preventing or reducing colonization of the gut by Salmonella Typhimurium was evaluated in pigs. A total of 63 4-week-old commercial piglets were randomly distributed into three different experimental dietary groups: a plain diet without additives (PD) and the same diet supplemented with either 0.4% (w/v) formic acid and 0.4% lactic acid (w/v) (AC) or 1% (w/v) lactulose (LC). After 7 days of adaptation, two-thirds of the pigs (14 from each diet) were challenged with a 2-mL oral dose of 10(8)CFU/mL of Salmonella Typhimurium, leaving the remaining animals unchallenged (UC). After 4 and 10 days post-challenge, pigs were euthanized and the ileum and caecum content were aseptically sampled to (a) quantify lactic, formic, and short-chain fatty acids (SCFA), (b) quantify bacterial populations and Salmonella by fluorescence in situ hybridization and (c) qualitatively analyse bacterial populations through denaturing gradient gel electrophoresis (DGGE). Modification of fermentation products and counts of some of the bacterial groups analysed in the challenged pigs receiving the treatments AC and LC were minimal. Treatments only influenced the bacterial diversity after 10 days post-challenge, with AC generating a lower number of DGGE bands than UC (P<0.05). Neither the inclusion of a mixture of 0.4% (w/v) formic and 0.4% (w/v) lactic acids nor of 1% (w/v) lactulose in the feed influenced numbers of Salmonella in the ileum and caecum of experimentally challenged pigs.
Authors:
Sandra Martín-Peláez; Adele Costabile; Lesley Hoyles; Robert A Rastall; Glenn R Gibson; Roberto M La Ragione; Martin J Woodward; Enric Mateu; Susana M Martín-Orúe
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-20
Journal Detail:
Title:  Veterinary microbiology     Volume:  142     ISSN:  1873-2542     ISO Abbreviation:  Vet. Microbiol.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-23     Completed Date:  2010-08-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705469     Medline TA:  Vet Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  337-45     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier B.V. All rights reserved.
Affiliation:
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona (UAB), Bellaterra, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biodiversity
Cecum / microbiology
Diet / veterinary*
Dietary Supplements*
Fatty Acids, Volatile / analysis
Formic Acids / administration & dosage*,  analysis
Gastrointestinal Contents / chemistry,  microbiology
Ileum / microbiology
Lactic Acid / administration & dosage*,  analysis
Lactulose / administration & dosage*
Male
Random Allocation
Salmonella Infections, Animal / microbiology,  prevention & control*
Salmonella typhimurium / growth & development
Swine
Swine Diseases / microbiology,  prevention & control*
Chemical
Reg. No./Substance:
0/Fatty Acids, Volatile; 0/Formic Acids; 4618-18-2/Lactulose; 50-21-5/Lactic Acid; 64-18-6/formic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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