Document Detail

Evaluation of the in Vivo Anti-inflammatory Effects of Extracts from Pyrus bretschneideri Rehd.
MedLine Citation:
PMID:  20672838     Owner:  NLM     Status:  In-Data-Review    
Pyrus bretschneideri Rehd., as a pharmaceutical supplement, is widely used in northern China to treat respiratory diseases. Our previous studies showed the ethanol extract of P. bretschneideri had significant anti-inflammatory activity. To isolate and identify the active ingredients, the ethanol extract was separated into petroleum ether, ethyl acetate, n-butanol, and aqueous fractions. The bioactivity of each fraction was investigated using an in vivo model. Results showed that the ethyl acetate fraction exhibited the strongest anti-inflammatory effect. Subsequently, this fraction was subjected to separation and purification using silica gel column chromatography, C18-ODS, and recrystallization, leading to two sterols and two triterpenes, which were identified as β-sitosterol, daucosterol, oleanolic acid, and ursolic acid. Moreover, all of the isolated compounds could significantly inhibit the ear edema induced by xylene. These results indicated that P. bretschneideri had good anti-inflammatory effects and the constituents β-sitosterol, daucosterol, oleanolic acid, and ursolic acid might well account for it.
Li-Jing Huang; Wen-Yuan Gao; Xia Li; Wan-Shun Zhao; Lu-Qi Huang; Chang-Xiao Liu
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Publication Detail:
Type:  Journal Article     Date:  2010-07-30
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  58     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2013-05-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8983-7     Citation Subset:  IM    
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
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