Document Detail


Evaluation of immunotherapy in invasive candidiasis: antifungal activity and cytokine expression assays.
MedLine Citation:
PMID:  15888942     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Candida albicans and non-albicans Candida spp. can cause serious infections in hospitalized and immunocompromised patients. A large number of antifungal agents and immunomodulators have been developed and may interact with both polymorphonuclear and mononuclear phagocytes against blastoconidia and pseudohyphae of Candida spp. Blastoconidia are predominantly destroyed by phagocytosis, whereas pseudohyphal killing is initiated after the attachment of phagocytes to the cell wall of the organism and subsequent extracellular release of oxidative and nonoxidative metabolites. An array of methods to evaluate phagocytosis of blastoconidia, oxidative burst, expression of cytokines and chemokines in response to Candida spp., as well as candidacidal activity are described in this chapter.
Authors:
Maria Simitsopoulou; Emmanuel Roilides
Publication Detail:
Type:  Evaluation Studies; In Vitro; Journal Article    
Journal Detail:
Title:  Methods in molecular medicine     Volume:  118     ISSN:  1543-1894     ISO Abbreviation:  Methods Mol. Med.     Publication Date:  2005  
Date Detail:
Created Date:  2005-05-12     Completed Date:  2005-06-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101123138     Medline TA:  Methods Mol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  161-79     Citation Subset:  IM    
Affiliation:
Laboratory of Infectious Diseases, 3rd Department of Pediatrics, Aristotle University of Thessaloniki, Hippokration Hospital, Greece.
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MeSH Terms
Descriptor/Qualifier:
Animals
Candida albicans / immunology
Candidiasis / immunology,  microbiology,  therapy*
Cell Separation
Chemokines / biosynthesis,  genetics
Cytokines / biosynthesis*,  genetics
Enzyme-Linked Immunosorbent Assay / methods
Humans
Immunotherapy / methods*
Phagocytes / immunology,  metabolism
Phagocytosis
Rabbits
Respiratory Burst
Reverse Transcriptase Polymerase Chain Reaction
Superoxides / metabolism
Tetrazolium Salts
Thiazoles
Chemical
Reg. No./Substance:
0/Chemokines; 0/Cytokines; 0/Tetrazolium Salts; 0/Thiazoles; 11062-77-4/Superoxides; 298-93-1/thiazolyl blue

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