| Evaluation of the immunocytochemical method for amino acids. | |
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MedLine Citation:
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PMID: 2875230 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Free amino acids can be coupled to proteins by glutaraldehyde. Rabbits immunised with a bovine serum albumin-glutaraldehyde-amino acid conjugate form antibodies that recognise similar conjugates with brain proteins in glutaraldehyde-fixed tissue. Antisera raised against conjugated GABA (gamma-aminobutyrate), glutamate, aspartate, taurine, glutamine, or glycine were tested against a variety of small molecular compounds that had been fixed by glutaraldehyde to brain protein and immobilised on cellulose ester filters for processing together with the brain sections. This system permitted closely similar conditions for testing and immunocytochemistry. After removing antibodies against the carrier used for immunisation and against cross reacting amino acid conjugates the antisera showed a high specificity. The specific nature of the antisera was corroborated by solid phase adsorption to the homologous antigens and by inhibition experiments with free amino acids and amino acid-glutaraldehyde fixation complexes. After transection of the striatonigral pathway the ipsilateral substantia nigra was almost depleted of GABA-like immunoreactivity; this observation lends additional support to the selectivity of the GABA antiserum. A semiquantitative relation was established between the concentration of amino acid before fixation in a model system and the subsequent intensity of immunostaining. Similar model experiments suggested that the conjugation of an amino acid to brain protein with glutaraldehyde, and the immunoreactivity of the conjugates, may be significantly inhibited in the presence of high concentrations of other amino compounds. |
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Authors:
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O P Ottersen; J Storm-Mathisen; S Madsen; S Skumlien; J Strømhaug |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: Medical biology Volume: 64 ISSN: 0302-2137 ISO Abbreviation: Med. Biol. Publication Date: 1986 |
Date Detail:
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Created Date: 1986-09-30 Completed Date: 1986-09-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0417300 Medline TA: Med Biol Country: FINLAND |
Other Details:
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Languages: eng Pagination: 147-58 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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immunology,
metabolism* Animals Antigen-Antibody Reactions Aspartic Acid / immunology, metabolism Brain / metabolism Glutamates / immunology, metabolism Glutamic Acid Histocytochemistry / methods* Immunochemistry / methods* Rats Tissue Distribution gamma-Aminobutyric Acid / immunology, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Glutamates; 56-12-2/gamma-Aminobutyric Acid; 56-84-8/Aspartic Acid; 56-86-0/Glutamic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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