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Evaluation of hydropathy of amino acids from a comparison of their viscosities inside vesicles and on supported lipid bilayers.
MedLine Citation:
PMID:  22118892     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The viscosity of amino acids enclosed in giant lipid vesicles (η(out)) subjected to a shear flow near a solid surface has been studied using quartz crystal microbalance (QCM). This viscosity has been compared with shear viscosity for the different amino acids adsorbed on supported bilayers (SLBs) (η(in)) of the lipids on quartz. Using a first approximation of vesicles as model rigid spheres, the measured viscosities and the extent of deformation of vesicles observed using optical microscopy, two non-dimensional parameters: the reduced volume and the ratio of (η(in))/(η(out)) have been analyzed as a function of physical parameters: vesicle substrate distance (vesicle vs. supported lipid bilayers), vesicle size and their variation as a function of the viscosity. The kinematics of the vesicles with the amino acids compared with the shear at supported lipid bilayers seems to describe a reasonable hydropathy scale for the amino acids. The results show that there is a direct correlation between the above parameters and the polarity variations in amino acids suggesting that the viscous force may be an important parameter and should be taken into account in studies on membrane proteins interacting with cells and cell adhesion in flow chambers where cell membrane and the adhesive substrate are in relative motion.
Authors:
Kamatchi Sankaranarayanan; A Dhathathreyan
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-25
Journal Detail:
Title:  Colloids and surfaces. B, Biointerfaces     Volume:  -     ISSN:  1873-4367     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-11-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9315133     Medline TA:  Colloids Surf B Biointerfaces     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
Chemical Lab., CLRI (CSIR), Adyar, Chennai 600020, India.
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