Document Detail

Evaluation of human erythrocytes as model cells in photodynamic therapy.
MedLine Citation:
PMID:  15113118     Owner:  NLM     Status:  MEDLINE    
The role of erythrocytes as targets in photodynamic therapy is a controversially discussed topic in the literature. Therefore five different, but well known photosensitisers (three zinc phthalocyanines, tetrabenzoporphine and pheophorbide a delivered in liposomes were used for photodynamic treatment of human erythrocytes. The phototoxic effect on these cells showed pronounced differences. It was in the range: zinc phthalocyanine = pheophorbide a > tetrabenzoporphine >> zinc octa-n-alkyl phthalocyanines. Data from the zinc octa-n-alkyl phthalocyanines were compared with photodynamic effects within cutaneous cell lines, treated under the same experimental conditions. The results show that erythrocytes are unlikely to make good models for predicting the efficiency of the photosensitiser in general, and the same applies to cells other than erythrocytes and in vivo. Possible reasons could be differences in dye accumulation. However, erythrocytes may well serve as model cells to explore the cellular and molecular mechanisms of photodynamic treatment.
L Kaestner
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article    
Journal Detail:
Title:  General physiology and biophysics     Volume:  22     ISSN:  0231-5882     ISO Abbreviation:  Gen. Physiol. Biophys.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2004-04-28     Completed Date:  2004-08-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8400604     Medline TA:  Gen Physiol Biophys     Country:  Slovakia    
Other Details:
Languages:  eng     Pagination:  455-65     Citation Subset:  IM    
Norwegian Radiation Protection Authority, Department of Radiation Medicine, PO Box 55, 1332 Oslo, Norway.
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MeSH Terms
Cell Line, Tumor
Cell Survival / drug effects,  radiation effects
Cells, Cultured
Dose-Response Relationship, Radiation
Erythrocytes / drug effects*,  radiation effects*
Fibrosarcoma / drug therapy*
Keratinocytes / drug effects*,  radiation effects*
Photochemotherapy / methods*
Photosensitizing Agents / pharmacology*
Treatment Outcome
Reg. No./Substance:
0/Photosensitizing Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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