| Evaluation of human embryonic stem cell-derived hepatocyte-like cells for detection of CYP1A inducers. | |
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MedLine Citation:
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PMID: 22673034 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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There is a great deal of interest in differentiation of human embryonic stem cells (hESCs) into hepatocyte-like cells for application in pharmaceutical screening. Cytochrome P450 (CYP) 1A is involved in the metabolic activation of procarcinogenic compounds as well as in detoxification of drugs. We differentiated hESCs into hepatocyte-like cells (hESC-derived hepatocyte-like cells) and examined whether CYP1A was induced in these cells by typical inducers of CYP1A. hESC-derived hepatocyte-like cells expressed albumin, α-fetoprotein, CYP3A4, CYP3A7, CYP1A1, CYP1A2, and UDP-glucuronyl transferase (UGT) 1A1 mRNA. The levels of CYP1A1, CYP1A2, and UGT1A1 mRNA expression were increased by omeprazole and 3-methylcholanthrene. Furthermore, the enzyme activity of CYP1A was also increased by these compounds. In conclusion, hESC-derived hepatocyte-like cells are available for the detection of CYP1A inducers. |
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Authors:
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Hiroyuki Tsuchiya; Tamihide Matsunaga; Kaori Aikawa; Noboru Kamada; Katsunori Nakamura; Hinako Ichikawa; Katsunori Sasaki; Shigeru Ohmori |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-29 |
Journal Detail:
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Title: Drug metabolism and pharmacokinetics Volume: - ISSN: 1880-0920 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-6-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101164773 Medline TA: Drug Metab Pharmacokinet Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Pharmacy, Shinshu University Hospital. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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