Document Detail


Evaluation of the holoenzyme content of aromatic L-amino acid decarboxylase in brain and liver tissues.
MedLine Citation:
PMID:  1510658     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have re-evaluated the content of the holo-form of aromatic L-amino acid decarboxylase in rat tissues. Aromatic L-amino acid decarboxylase was found to consume pyridoxal 5'-phosphate while it underwent decarboxylation-dependent transamination as a side reaction. We observed that the total dopamine formation was proportional to the amount of holoenzyme. Dopamine formation in a tissue extract, which was preincubated with pyridoxal 5'-phosphate, was compared with the same tissue sample but which was prepared without preincubation. Percentages of holo-form of aromatic L-amino acid decarboxylase obtained from such comparison were 78% for brain and 94% for liver tissues. These values were significantly higher than those reported earlier in which the decarboxylation-dependent transamination of the decarboxylase had been overlooked.
Authors:
Y Kawasaki; H Hayashi; K Hatakeyama; H Kagamiyama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  186     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1992 Aug 
Date Detail:
Created Date:  1992-09-18     Completed Date:  1992-09-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1242-8     Citation Subset:  IM    
Affiliation:
Department of Medical Chemistry, Osaka Medical College, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoenzymes / analysis
Aromatic-L-Amino-Acid Decarboxylases / analysis*,  isolation & purification,  metabolism
Brain / enzymology*
Kinetics
Liver / enzymology*
Mathematics
Models, Biological
Rats
Rats, Inbred Strains
Chemical
Reg. No./Substance:
0/Apoenzymes; EC 4.1.1.28/Aromatic-L-Amino-Acid Decarboxylases
Comments/Corrections
Erratum In:
Biochem Biophys Res Commun 1992 Dec 15;189(2):1252

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