| Evaluation of hepatic changes and local and systemic immune responses in goats immunized with recombinant Peroxiredoxin (Prx) and challenged with Fasciola hepatica. | |
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MedLine Citation:
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PMID: 20153792 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Protection against Fasciola hepatica in goats immunized with Peroxiredoxin (Prx) was assessed. The experimental trial consisted of three groups of seven animals; group 1 were unimmunized and uninfected, group 2 were immunized with adjuvant only and group 3 were immunized with recombinant Prx in adjuvant (immunized and infected). Immunization with Prx in Quil A adjuvant, group 3, induced a reduction in fluke burden of 33.04% when compared to adjuvant control, group 2, although this difference was not significant. The hepatic gross and microscopical morphometric study revealed lower damage in the Prx-immunized compared to group 2 (p<0.05). Furthermore, immunohistochemical studies revealed that the Prx-immunized group exhibited reduced infiltration of CD4(+), CD8(+), IFN-gamma(+) and TCR(+) (p<0.05); and CD2(+) and IL-4(+) (p<0.001) in hepatic lesions. Levels of anti-Prx serum IgG in group 3 showed a significant increase at the 4th week after challenge infection compared with group 2 (p<0.0001). This is the first report of ruminant immunization with recombinant Prx of F. hepatica. The study shows that this vaccine significantly reduces hepatic damage and encourages further studies to improve the vaccine efficacy. |
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Authors:
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Ricardo E Mendes; Rafael A P?rez-Ecija; Rafael Zafra; Leandro Buffoni; Alvaro Mart?nez-Moreno; John P Dalton; Grace Mulcahy; Jos? P?rez |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-12 |
Journal Detail:
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Title: Vaccine Volume: 28 ISSN: 1873-2518 ISO Abbreviation: Vaccine Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-29 Completed Date: 2010-06-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406899 Medline TA: Vaccine Country: Netherlands |
Other Details:
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Languages: eng Pagination: 2832-40 Citation Subset: IM |
Copyright Information:
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(c) 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Anatomy and Comparative Pathology, School of Veterinary Medicine, University of Cordoba, Animal Health Building, Campus Rabanales, Cordoba, Spain. z62memer@uco.es |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adjuvants, Immunologic
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administration & dosage Animals Antibodies, Helminth / blood Antigens, CD2 / analysis CD4-Positive T-Lymphocytes / immunology CD8-Positive T-Lymphocytes / immunology Fasciola hepatica / immunology* Fascioliasis / immunology, pathology, prevention & control, veterinary* Goat Diseases / immunology, pathology, prevention & control* Goats Immunoglobulin G / blood Immunohistochemistry Interferon-gamma / analysis Interleukin-4 / analysis Liver / parasitology, pathology* Peroxiredoxins / immunology* Receptors, Antigen, T-Cell / analysis Saponins / administration & dosage Vaccination / methods Vaccines / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Adjuvants, Immunologic; 0/Antibodies, Helminth; 0/Antigens, CD2; 0/Immunoglobulin G; 0/Receptors, Antigen, T-Cell; 0/Saponins; 0/Vaccines; 207137-56-2/Interleukin-4; 66594-14-7/Quil A; 82115-62-6/Interferon-gamma; EC 1.11.1.14/peroxiredoxin, Fasciola hepatica; EC 1.11.1.15/Peroxiredoxins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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