Document Detail


Evaluation of a green laser pointer for flow cytometry.
MedLine Citation:
PMID:  17712796     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Flow cytometers typically incorporate expensive lasers with high-quality (TEM00) output beam structure and very stable output power, significantly increasing system cost and power requirements. Red diode lasers minimize power consumption and cost, but limit fluorophore selection. Low-cost DPSS laser pointer modules could possibly offer increased wavelength selection but presumed emission instability has limited their use. A $160 DPSS 532 nm laser pointer module was first evaluated for noise characteristics and then used as the excitation light source in a custom-built flow cytometer for the analysis of fluorescent calibration and alignment microspheres. Eight of ten modules tested were very quiet (RMS noise < or = 0.6% between 0 and 5 MHz). With a quiet laser pointer module as the light source in a slow-flow system, fluorescence measurements from alignment microspheres produced CVs of about 3.3%. Furthermore, the use of extended transit times and < or =1 mW of laser power produced both baseline resolution of all 8 peaks in a set of Rainbow microspheres, and a detection limit of <20 phycoerythrin molecules per particle. Data collected with the transit time reduced to 25 micros (in the same instrument but at 2.4 mW laser output) demonstrated a detection limit of approximately 75 phycoerythrin molecules and CVs of about 2.7%. The performance, cost, size, and power consumption of the tested laser pointer module suggests that it may be suitable for use in conventional flow cytometry, particularly if it were coupled with cytometers that support extended transit times.
Authors:
Robert C Habbersett; Mark A Naivar; Travis A Woods; Gregory R Goddard; Steven W Graves
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Cytometry. Part A : the journal of the International Society for Analytical Cytology     Volume:  71     ISSN:  1552-4922     ISO Abbreviation:  Cytometry A     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-09-26     Completed Date:  2007-12-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101235694     Medline TA:  Cytometry A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  809-17     Citation Subset:  IM    
Affiliation:
The National Flow Cytometry Resource, Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
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MeSH Terms
Descriptor/Qualifier:
Flow Cytometry / economics,  methods*
Fluorescence
Lasers*
Microspheres
Oxazines / metabolism
Time Factors
Grant Support
ID/Acronym/Agency:
RR001315/RR/NCRR NIH HHS; RR020064/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Oxazines; 7385-67-3/nile red

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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