| Evaluation of a green laser pointer for flow cytometry. | |
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MedLine Citation:
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PMID: 17712796 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Flow cytometers typically incorporate expensive lasers with high-quality (TEM00) output beam structure and very stable output power, significantly increasing system cost and power requirements. Red diode lasers minimize power consumption and cost, but limit fluorophore selection. Low-cost DPSS laser pointer modules could possibly offer increased wavelength selection but presumed emission instability has limited their use. A $160 DPSS 532 nm laser pointer module was first evaluated for noise characteristics and then used as the excitation light source in a custom-built flow cytometer for the analysis of fluorescent calibration and alignment microspheres. Eight of ten modules tested were very quiet (RMS noise < or = 0.6% between 0 and 5 MHz). With a quiet laser pointer module as the light source in a slow-flow system, fluorescence measurements from alignment microspheres produced CVs of about 3.3%. Furthermore, the use of extended transit times and < or =1 mW of laser power produced both baseline resolution of all 8 peaks in a set of Rainbow microspheres, and a detection limit of <20 phycoerythrin molecules per particle. Data collected with the transit time reduced to 25 micros (in the same instrument but at 2.4 mW laser output) demonstrated a detection limit of approximately 75 phycoerythrin molecules and CVs of about 2.7%. The performance, cost, size, and power consumption of the tested laser pointer module suggests that it may be suitable for use in conventional flow cytometry, particularly if it were coupled with cytometers that support extended transit times. |
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Authors:
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Robert C Habbersett; Mark A Naivar; Travis A Woods; Gregory R Goddard; Steven W Graves |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Cytometry. Part A : the journal of the International Society for Analytical Cytology Volume: 71 ISSN: 1552-4922 ISO Abbreviation: Cytometry A Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-09-26 Completed Date: 2007-12-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101235694 Medline TA: Cytometry A Country: United States |
Other Details:
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Languages: eng Pagination: 809-17 Citation Subset: IM |
Affiliation:
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The National Flow Cytometry Resource, Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Flow Cytometry
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economics,
methods* Fluorescence Lasers* Microspheres Oxazines / metabolism Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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RR001315/RR/NCRR NIH HHS; RR020064/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Oxazines; 7385-67-3/nile red |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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