| Evaluation of glucuronide metabolite stability in dried blood spots. | |
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MedLine Citation:
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PMID: 23216122 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background: Stabilization of phase II metabolites is an important consideration during bioanalytical method development, method validation and sample analysis. Generic approaches to stabilize these metabolites during storage in liquid-based matrices include pH adjustment of samples prior to storage and/or temperature control; although a variety of other compound-specific stabilization techniques exist. Dried blood spot (DBS) technology is becoming a popular alternative to liquid matrix sampling in many preclinical and clinical applications. However, concerns remain regarding the stability of metabolites stored under ambient conditions using DBS. Results: Experimental data have shown that, under ambient storage conditions, the stability of the glucuronides investigated herein stored as DBS is equivalent to that of liquid samples stored at -80°C. Conclusion: The decision to employ DBS technology for a given study needs to be considered on a case-by-case basis with an understanding of compound-specific metabolism characteristics and clinical study design. |
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Authors:
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Chester L Bowen; Jonathan Volpatti; Jessica Cades; Hermes Licea-Perez; Christopher A Evans |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Bioanalysis Volume: 4 ISSN: 1757-6199 ISO Abbreviation: Bioanalysis Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-12-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101512484 Medline TA: Bioanalysis Country: England |
Other Details:
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Languages: eng Pagination: 2823-32 Citation Subset: IM |
Affiliation:
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Platform Technology & Science, Drug Metabolism & Pharmacokinetics, Bioanalytical Science & Toxicokinetics, GlaxoSmithKline Pharmaceuticals, 709 Swedeland Road, King of Prussia, PA 19406, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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