Document Detail


Evaluation of genetic variability in in vitro sodium azide-induced Dendrobium 'Earsakul' mutants.
MedLine Citation:
PMID:  25078589     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
In vitro mutagenesis of Dendrobium 'Earsakul' was carried out by incubating the protocorm-like bodies in 0-5 mM sodium azide for 1 h. Twenty-eight putative mutants were evaluated for genetic variability compared to untreated control plants using inter-simple sequence repeat (ISSR) analysis. Polymorphic fragments were produced by 9 of 12 ISSR primers. A total of 173 amplified ISSR fragments varying in size from 140 to 5000 bp were obtained, 39 of which were polymorphic (22.5%). Of the 28 putative mutants, 15 (53.6%) showed altered genetic profiles compared to control and were identified as mutants. These results suggest that sodium azide can be effectively utilized to generate mutants in Dendrobium 'Earsakul', and ISSR provides a powerful tool that allows efficient early detection of these mutants. The identified mutants are currently being multiplied for further evaluation of their horticultural characteristics.
Authors:
A Wannajindaporn; O Poolsawat; W Chaowiset; P A Tantasawat
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Publication Detail:
Type:  Journal Article     Date:  2014-07-24
Journal Detail:
Title:  Genetics and molecular research : GMR     Volume:  13     ISSN:  1676-5680     ISO Abbreviation:  Genet. Mol. Res.     Publication Date:  2014  
Date Detail:
Created Date:  2014-08-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101169387     Medline TA:  Genet Mol Res     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  5333-42     Citation Subset:  IM    
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