| Evaluation of a fluid versus a powder pepsin formulation to detect Trichinella spiralis larvae in meat samples by a digestion technique. | |
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MedLine Citation:
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PMID: 18095451 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pepsin powder constitutes a health risk, potentially causing severe allergic reactions to those handling the chemical. A fluid pepsin formulation was produced and tested, first in a preliminary study and then in a ring trial encompassing four European National Reference Laboratories (NRLs). The purpose of each trial was to ascertain and compare the action of pepsin powder with that of the pepsin fluid for digesting meat and liberating encapsulated Trichinella spiralis larvae for subsequent counting. The quality of digestion was furthermore evaluated by assessing the visibility through the digestion fluid and the amount of debris remaining after digestion. For the ring trial, at each laboratory 20 blinded replicate 100-g samples of pork meat containing a known number of encapsulated T. spiralis larvae (0 to 30) were digested by the magnetic stirrer method using either the standard pepsin powder (10 samples) or the pepsin fluid (10 samples). With an average recovery rate of 70 to 80%, all NRLs found the pepsin fluid and pepsin powder to be equally effective. The NRLs also found no difference between the two pepsin formulations with regard to debris remnants or visibility through the digestion fluid. The use of pepsin fluid may therefore constitute an improvement of the digestion procedure for the analysts involved. |
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Authors:
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Charlotte Maddox-Hyttel; Karsten Nöckler; Edoardo Pozio; Isabelle Vallée; Pascal Boireau |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of food protection Volume: 70 ISSN: 0362-028X ISO Abbreviation: J. Food Prot. Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-12-21 Completed Date: 2008-04-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7703944 Medline TA: J Food Prot Country: United States |
Other Details:
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Languages: eng Pagination: 2896-9 Citation Subset: IM |
Affiliation:
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Department for Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, DK-1790 Copenhagen V, Denmark. cmh@vet.dtu.dk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Consumer Product Safety Digestion Food Contamination / analysis* Food Parasitology* Gastrointestinal Agents / pharmacology Humans Laboratory Techniques and Procedures / standards* Meat / parasitology* Parasite Egg Count Pepsin A / pharmacology* Powders Trichinella spiralis / isolation & purification* |
| Chemical | |
Reg. No./Substance:
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0/Gastrointestinal Agents; 0/Powders; EC 3.4.23.1/Pepsin A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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