Document Detail


Evaluation of a flash disinfection process for surface decontamination of gamma-irradiated feed packaging.
MedLine Citation:
PMID:  17645295     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ensuring biosecurity for pathogen-free rodents generally requires processing all materials that come in direct contact with the animals, including feed, to reduce or eliminate unwanted adventitious agents. A common method of processing animal feed is gamma irradiation. Irradiation is performed offsite and requires transport of feed from the irradiator to the point of use, potentially resulting in surface contamination of the packaging. We tested whether an autoclave could be used to provide a flash disinfection cycle to decontaminate the outer feed packaging while having a limited effect on nutritional feed quality. We developed a standardized and repeatable method, which involved attaching sterile glass vials containing Pseudomonas aeruginosa- and Staphylococcus aureus-laden culture broth onto the bag's surface, to validate effectiveness of the process. Nutritional analyses verified that the flash process had minimal effect on feed quality. Gas chromatography-mass spectrometry confirmed that subjecting feed packaging to the elevated cycle temperatures and pressures did not result in feed contamination by the packaging materials. The lowest autoclave setting that produced consistent surface disinfection, as determined by 3 consecutive negative cultures, was exposure of the bag surface to a chamber temperature of at least 82 degrees C for a minimum of 2 min. This flash disinfection process has been implemented successfully in 5 vivaria supporting more than 35,000 rodent cages daily.
Authors:
Ralph W Thurlow; Raquel Arriola; Clifford E Soll; Neil S Lipman
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Journal of the American Association for Laboratory Animal Science : JAALAS     Volume:  46     ISSN:  1559-6109     ISO Abbreviation:  J. Am. Assoc. Lab. Anim. Sci.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-24     Completed Date:  2007-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101269489     Medline TA:  J Am Assoc Lab Anim Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  46-9     Citation Subset:  IM    
Affiliation:
Research Animal Resource Center, Memorial Sloan-Kettering Cancer Center and the Weill Medical College of Cornell University, New York, NY, USA.
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MeSH Terms
Descriptor/Qualifier:
Animal Feed / analysis,  microbiology*,  radiation effects
Disinfection / methods*,  standards
Food Contamination / analysis,  prevention & control*
Food Packaging*
Gamma Rays*
Gas Chromatography-Mass Spectrometry

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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